Highlights:

• Forms covalent bonds with sulfhydryl groups at pH 6.5-7.5
• Reaction of the maleimide group with sulfhydryls can be monitored by measuring the decrease in absorbance at 300 nm
• Has been used successfully for the blocking of sulfhydryl containing reagents that interfere in enzyme assays

References:

1. Partis, M.D., et al. (1983) J. Prot. Chem. 2(3), 263-277.