Stop proteolysis fast with a cocktail that is delivered to your sample within seconds

Thermo Scientific Halt Protease Inhibitor Cocktails provide all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0.5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents.

Convenient package sizes and formats

Halt Protease Inhibitor Cocktails are available in 100 µl (pack of 24), 5 ml, 10 ml aliquots, providing enough reagent to treat up to 1 L of cell extract. The 5 ml and 10 ml package sizes are ideal for larger applications while the 24 x 100 µl format preserves the integrity of the formulation between infrequent uses by preventing the reagent from exposure to air, moisture and other potential contamination.

Users of protease inhibitor cocktails often need to know the exact details pertaining to the cocktail formulation. Many manufacturers hold their formulations proprietary and will not disclose composition or concentrations. Halt Protease Inhibitor Cocktails can be used with confidence because the identity and concentration of each component of the cocktail is readily available. Furthermore, Halt’s EDTA-free formulation is ideal for preparing samples that will be analyzed by 2-D gel electrophoresis, immobilized metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Highlights of Halt Protease Inhibitor Cocktails

• Compatible with Thermo Scientific Cell Lysis Buffers
  • Effective for suppressing proteolytic activity in detergent-based cell lysis reagents
  • Outstanding performance demonstrated with Thermo Scientific M-PER Mammalian, B-PER Bacterial, T-PER Tissue and Y-PER Yeast Protein Extraction Reagents  
• Conventient packaging
  • Single-Use packaging is ideal for small scale or infrequent use
  • 5 ml and 10 ml sizes available for increased use
• EDTA-free formulation
  • Metalloproteases may be inhibited by addition of the optional EDTA stock solution
  • EDTA-free formulation inhibits proteolytic activity (except metalloproteases) in applications where EDTA may affect protein stability and function
  • Omit EDTA to prevent interference with subsequent assays or purification methods such as immobilized metal chelate affinity chromatography (IMAC)
• Stable refrigerated stock solution
  • Save valuable freezer space
  • No waiting for tablets to completely dissolve
  • Easly dispense the correct amount of inhibitor

• Protection of intact, active cellular proteins from degradation by endogenous proteases
• Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Cell Lysis Reagents, other commercially formulated cell or tissue lysis products, sonication, French press, blender homogenization or repetitive freeze/thaw cycling.
• Screening of extracts for proteolytic activity.
• The study of proteolysis in the regulation of cellular processes

Thermo Scientific Halt Protease Inhibitor Single-use Cocktails are more effective than tablet-format cocktails. Using a validated protease assay and 1.0 mg/ml of rat pancrease exrtract, the Halt Protease Inhibitor Single-use Cocktail, with and without EDTA added, were tested against commercially available tablet-format protease inhibitor cocktails under the same conditions. A 1X final concentration of each inhibitor was added. The single-use formulation resulted in ≥ 97% inhibition compared to ≥ 59% inhibition for the tablet.

References to Applications of Protease Inhibitor Cocktails

1. Kulakowska-Bodzon, A. et al. (2006). Methods for samples preparation in proteomic research. J. Chrom. B (doi:10.1016/j.jchromb.2006.10.040).
2. North, M.J. (1989). Prevention of unwanted proteolysis in Proteolytic Enzymes: A Practical Approach (Beynon, R.J., Bond, J.S. eds), pp. 105-124, IRL Press, Oxford.
3. Salvensen, G. and Nagase, H. (1989). Inhibition of proteolytic enzymes in Proteolytic Enzymes: A Practical Approach (Beynon, R.J., Bond, J.S. eds). pp. 83-104, IRL Press, Oxford.

Related Resources
Halt Protease Inhibitors FAQ
Protein Methods Library: Protease and Phosphatase Inhibitors

Related Products
Halt Phosphatase Inhibitor Cocktails
Halt Protease and Phosphatase Inhibitor Cocktails
M-PER Mammalian Protein Extraction Reagent
B-PER Protein Extraction Reagents
All Pierce Cell Lysis Solutions

Halt Protease Inhibitors are available in bulk quantities.