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YOU ARE HERE:  Browse Product Catalog > Cell Lysis and Protein Extraction > Protease Inhibitors and Phosphatase Inhibitors > PMSF  


PMSF (phenylmethylsulfonyl fluoride) 
Reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.

PMSF phenylmethylsulfonyl fluoride M.W. 174.19

Reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.

In addition to inhibiting serine proteases, PMSF will also inhibit cysteine proteases, like papain (reversible by DTT treatment), and mammalian aceylcholinesterase.

Highlights:

  • High salt concentrations are used to reverse the binding (and inhibition) of PMSF to enzymes.
  • Because PMSF has limited water solubility, it must be dissolved in a small amount of solvent, ethanol or methanol before addition to a buffer.
  • Targets serine proteases
  • Dissolve in a small amount of solvent, ethanol or methanol before addition
    to a buffer

Specifications :
Formula : C6H5CH2SO2F
Molecular weight: 174.20
Appearance : white powder
Purity : > 99.0 %
Melting point : 92-95°C
Humidity : < 0.1 %

Synonyms: alpha-Toluenesulfonyl fluoride
Benzylsulfonyl fluoride
Phenylmethylsulfonyl fluoride

CAS Number 329-98-6
EC Number: 206-350-2

Recommended storage conditions : Room Temperature in a dry place and protected from light.

Working solution:
Dissolve  PMSF in isopropanol  at  1.74 mg/ml (10mM).
Store this solution in aliquots at –20°C.

R : 25-34
S : 26-36/37/39-45

Related Pierce Products:
Halt Protease Inhibitor Cocktail

* PMSF is available in bulk quantities for manufacturing applications.

Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 36978 PMSF (phenylmethylsulfonyl fluoride) 5 g MSDS for product # 36978  PMSF (phenylmethylsulfonyl fluoride) $56.00

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