Reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.

In addition to inhibiting serine proteases, PMSF will also inhibit cysteine proteases, like papain (reversible by DTT treatment), and mammalian aceylcholinesterase.

Highlights:

• High salt concentrations are used to reverse the binding (and inhibition) of PMSF to enzymes.
• Because PMSF has limited water solubility, it must be dissolved in a small amount of solvent, ethanol or methanol before addition to a buffer.
• Targets serine proteases
• Dissolve in a small amount of solvent, ethanol or methanol before addition
  to a buffer

Specifications :
Formula : C₆H₅CH₂SO₂F
Molecular weight: 174.20
Appearance : white powder
Purity : > 99.0 %
Melting point : 92-95°C
Humidity : < 0.1 %

CAS Number 329-98-6
EC Number: 206-350-2

Recommended storage conditions : Room Temperature in a dry place and protected from light.

Working solution:
Dissolve  PMSF in isopropanol  at  1.74 mg/ml (10mM).
Store this solution in aliquots at –20°C.

R : 25-34
S : 26-36/37/39-45

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