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Amino Acid Side Chain Modification Agents  |
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Applications in reversible immobilization and crosslinking.
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Converts free sulfhydryls into primary amine groups.
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A photoreactive, iodinatable and carbonyl-reactive crosslinker.
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Modifies sulfhydryl groups to carboxyl groups for the preparation of peptide-protein conjugates.
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Citraconic Anhydride (2-methylmaleic anhydride)reversibly blocks primary amines at pH 8. Amines can be unblocked and returned to their native form.
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Ethylene diamine is often used with the crosslinker EDC to convert carboxyl groups into terminal amines. This process blocks the carboxyl groups and changes the charge of the protein.
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HPG reacts specifically with arginine residues.
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Hydroxylamine HCl is a highly effective reagent for deblocking SATA and SATP modified proteins to generate a free sulfhydryl.
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Methyl methanethiosulfonate is a sulfhydryl-reactive and reversible stet reagent.
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Amine-reactive with latent carboxyl group. Converts amines to protected carboxyls. Carboxyls are unmasked at ph 9.5 in phosphate buffer.
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Useful for permanently blocking free sulfhydryls.
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An oxidation reagent of choice for creating active aldehydes from carbohydrates for use in solid phase and solution coupling strategies.
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These reagents react with primary amines to add protected sulfhydryls. SATA, SATP and the new SAT(PEO4) are used to convert primary amines to sulfhydryls with varying spacer arms.
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A water-soluble acylating reagent that blocks primary amines at pH 7 or greater.
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A water-soluble reagent that reacts with primary amines at pH 7-10 to introduce sulfhydryl groups.
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