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AEDP
Aminoethylate Reagent
ASBA
BMPA
Citraconic Anhydride
Ethylenediamine dihydrochloride
HPG
Hydroxylamine-HCl
Iodoacetic Acid
MMTS
MSA
N-Ethylmaleimide (NEM)
SAT(PEG)4
SATA and SATP
Sodium meta-Periodate
Sulfhydryl Addition Kit
Sulfo-NHS-Acetate
TFCS
Traut's Reagent
EMCA

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Modification Reagents for Amino Acids 

Chemical agents to modify amino acid side chains on proteins and peptides in order to alter native charges, block or expose reactive binding sites, inactivate functions, or change functional groups to create targets for crosslinking and labeling.


Applications in reversible immobilization and crosslinking. 
 

Converts free sulfhydryls into primary amine groups. 
 

A photoreactive, iodinatable and carbonyl-reactive crosslinker.  
 

Modifies sulfhydryl groups to carboxyl groups for the preparation of peptide-protein conjugates.  
 

Citraconic Anhydride (2-methylmaleic anhydride)reversibly blocks primary amines at pH 8. Amines can be unblocked and returned to their native form. 
 

Ethylene diamine is often used with the crosslinker EDC to convert carboxyl groups into terminal amines. This process blocks the carboxyl groups and changes the charge of the protein. 
 

HPG reacts specifically with arginine residues. 
 

Hydroxylamine HCl is a highly effective reagent for deblocking SATA and SATP modified proteins to generate a free sulfhydryl. 
 

For carboxymethylation.  
 

Methyl methanethiosulfonate is a sulfhydryl-reactive and reversible stet reagent.  
 

Amine-reactive with latent carboxyl group. Converts amines to protected carboxyls. Carboxyls are unmasked at ph 9.5 in phosphate buffer. 
 

Covalently blocks sulfhydryl groups at pH 6.5-7.5 and the reaction of the maleimide group with sulfhydryls can be monitored by measuring the decrease in absorbance at 300nm. 
 

Converts amine groups to sulfhydryl groups and provides the solubility benefits associated with polyethylene glycol (PEG) containing reagents. 
 

Sulfhydryl-addition reagents that react with primary amines to add protected sulfhydryls. 
 

An oxidation reagent of choice for creating active aldehydes from carbohydrates for use in solid phase and solution coupling strategies.  
 

This kit introduces free sulfhydryl groups (-SH) into a protein of interest. These groups act as a handle to allow reaction of the modified protein with a variety of sulfhydryl-selective products. 
 

A water-soluble acylating reagent that blocks primary amines at pH 7 or greater.  
 

Amine-reactive crosslinker with a latent amino group available on demand.  
 

A water-soluble reagent that reacts with primary amines at pH 7-10 to introduce sulfhydryl groups.  
 

Useful for introducing maleimide groups into biomolecules. 
 

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