Modification Reagents for Amino Acids


	AEDP Applications in reversible immobilization and crosslinking.


	Aminoethylate Reagent Converts free sulfhydryls into primary amine groups.


	ASBA A photoreactive, iodinatable and carbonyl-reactive crosslinker.


	BMPA Modifies sulfhydryl groups to carboxyl groups for the preparation of peptide-protein conjugates.


	Citraconic Anhydride Citraconic Anhydride (2-methylmaleic anhydride)reversibly blocks primary amines at pH 8. Amines can be unblocked and returned to their native form.


	Ethylenediamine dihydrochloride Ethylene diamine is often used with the crosslinker EDC to convert carboxyl groups into terminal amines. This process blocks the carboxyl groups and changes the charge of the protein.


	HPG HPG reacts specifically with arginine residues.


	Hydroxylamine-HCl Hydroxylamine HCl is a highly effective reagent for deblocking SATA and SATP modified proteins to generate a free sulfhydryl.


	Iodoacetic Acid For carboxymethylation.


	MMTS Methyl methanethiosulfonate is a sulfhydryl-reactive and reversible stet reagent.


	MSA Amine-reactive with latent carboxyl group. Converts amines to protected carboxyls. Carboxyls are unmasked at ph 9.5 in phosphate buffer.


	N-Ethylmaleimide (NEM) Covalently blocks sulfhydryl groups at pH 6.5-7.5 and the reaction of the maleimide group with sulfhydryls can be monitored by measuring the decrease in absorbance at 300nm.


	SAT(PEG)4 Converts amine groups to sulfhydryl groups and provides the solubility benefits associated with polyethylene glycol (PEG) containing reagents.


	SATA and SATP Sulfhydryl-addition reagents that react with primary amines to add protected sulfhydryls.


	Sodium meta-Periodate An oxidation reagent of choice for creating active aldehydes from carbohydrates for use in solid phase and solution coupling strategies.


	Sulfhydryl Addition Kit This kit introduces free sulfhydryl groups (-SH) into a protein of interest. These groups act as a handle to allow reaction of the modified protein with a variety of sulfhydryl-selective products.


	Sulfo-NHS-Acetate A water-soluble acylating reagent that blocks primary amines at pH 7 or greater.


	TFCS Amine-reactive crosslinker with a latent amino group available on demand.


	Traut's Reagent A water-soluble reagent that reacts with primary amines at pH 7-10 to introduce sulfhydryl groups.


	EMCA Useful for introducing maleimide groups into biomolecules.


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