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Thermo Scientific - Pierce Protein Research Products  
Crosslinkers at a Glance
Crosslinker Selection Guide
Homobifunctional Crosslinkers
Heterobifunctional Crosslinkers
NHS and Sulfo-NHS
Deuterated Crosslinkers for Mass Spec Analysis
Photoreactive Amino Acids
Azide-Phosphine Staudinger Ligation Pairs
Azide-Phosphine Staudinger Reagent Guide
Bioconjugate Toolkit Reagents

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YOU ARE HERE:  Browse Product Catalog > Crosslinking and Protein Modification > Crosslinking Reagents  



Crosslinking Reagents 

What is cross-linking?
Cross-linking is the process of chemically joining two molecules by a covalent bond.  Cross-linking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules.  Several chemical groups that may be targets for reactions in proteins and peptides are readily available, allowing them to be easily conjugated and studied using cross-linking methods.  Cross-linkers also are commonly used to modify nucleic acids, drugs, solid surfaces and other molecules.

How are chemical cross-linkers used?
  • Chemical cross-linking agents are used to determine near-neighbor relationships (BS3), analyze protein structure and provide information on the distance between interacting molecules ( Isotope labeled Crosslinkers).
  • Cross-linkers are used to prepare antibody-enzyme conjugates ( Sulfo-SMCC), immobilize molecules ( EDC , Sulfo-NHS) and conjugate haptens to carrier proteins (Sulfo-SMCC , EDC).

Crosslinkers at a Glance 
A quick reference guide to Pierce Crosslinking Reagents, listing all products by target reactivity and chemical properties. 


Crosslinker Selection Guide 
An interactive guide to Pierce Crosslinking Reagents! You supply the critical parameters and we supply the list of the appropriate crosslinkers. 


Homobifunctional Crosslinkers 
Crosslinkers with identical reactive groups at either end, enabling one-step conjugation between like functional groups in proteins and other molecules. 


Heterobifunctional Crosslinkers 
Crosslinkers with different reactive groups at either end, enabling sequential conjugation between two different functional groups in proteins and other molecules. 


NHS and Sulfo-NHS 
The essential building blocks for amine-reactive activation of carboxylate molecules with EDC. Create amine-reactive NHS ester compounds or facilitate carbodiimide-mediated carboxyl-to-amine conjugations. 


Deuterated Crosslinkers for Mass Spec Analysis 
Matched pairs of deuterated (heavy) and non-deuterated (light) crosslinkers to assist in protein structural analysis by mass spectrometry (MS). 


Photoreactive Amino Acids 
Photo-reactive in vivo Crosslinking with Photo-Leucine and Photo-Methionine 


Azide-Phosphine Staudinger Ligation Pairs 
Azide and phosphine compounds activated as NHS esters for conjugation to amine-containing proteins and surfaces.  


Azide-Phosphine Staudinger Reagent Guide 
Menu of Staudinger chemoselective ligation reagents, including bioorthogonal azide metabolic labeling reagents, azide-phosphine crosslinker pairs and phosphine-activated fluors and biotin. 


Bioconjugate Toolkit Reagents 
Reagent pairs of heterobifunctional crosslinkers for attaching purified proteins in a controlled manner. Each amine-reactive reagent contains an NHS ester and either a hydrazine/hydrazide or aldehyde group. 

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