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What is cross-linking?
Cross-linking is the process of chemically joining two molecules by a covalent bond. Cross-linking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. Several chemical groups that may be targets for reactions in proteins and peptides are readily available, allowing them to be easily conjugated and studied using cross-linking methods. Cross-linkers also are commonly used to modify nucleic acids, drugs, solid surfaces and other molecules.
How are chemical cross-linkers used?
- Chemical cross-linking agents are used to determine near-neighbor relationships (BS3), analyze protein structure and provide information on the distance between interacting molecules ( Isotope labeled Crosslinkers).
- Cross-linkers are used to prepare antibody-enzyme conjugates ( Sulfo-SMCC), immobilize molecules ( EDC , Sulfo-NHS) and conjugate haptens to carrier proteins (Sulfo-SMCC , EDC).
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