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YOU ARE HERE:  Browse Product Catalog > Protein Assays > Glycoprotein Carbohydrate Estimation Kit  


Glycoprotein Carbohydrate Estimation Kit 

Direct approach for estimation of protein carbohydrate content.

Carbohydrate Estimation Kit Glycoprotein StandardsThe Thermo Scientific Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Use the kit to verify whether a purified antibody is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents.

Highlights:

  • Easily identify a purified protein as a glycoprotein
  • Estimate the percent carbohydrate content of a glycoprotein by comparison to the included set of glycoprotein standards
  • Determines carbohydrate content in three easy steps: (1) oxidize, (2) react and (3) measure the absorbance
  • Entire assay performed in less than 75 minutes
  • All you need is this kit, a microplate and a plate reader to determine carbohydrate content

How the Assay Measures Carbohydrate Content:

The protein sample under analysis is oxidized and reacted with the exclusive Glycoprotein Detection Reagent. The resulting colored complex is read at 550 nm. From the absorbance of the resulting complex at 550 nm the approximate percentage of carbohydrate in the glycoprotein under analysis can be estimated.

Related Products and Resources:
Glycoprotein Carbohydrate Estimation Kit FAQ
Protein Assay Selection Guide
Pierce Glycoprotein Staining Kit
Krypton Fluorescent Glycoprotein Staining Kit
Glycoprotein Isolation Kits


Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 23260 Glycoprotein Carbohydrate Estimation Kit
Sufficient reagents for 250 microplate assays or 60 standard test tube assays.

Kit Contents:
Sodium meta-Periodate, 500mg
Glycoprotein Detection Reagent, 500mg
Glycoprotein Assay Buffer, 250mL
Lyophilized Glycoprotein Standard Set, 1 kit
Kit MSDS for product # 23260 Glycoprotein Carbohydrate Estimation Kit Product Instructions $312.00
Add 23262 Glycoprotein Detection Reagent 1 g Product Instructions MSDS for product # 23262 Glycoprotein Detection Reagent $58.00
Add 23259 Lyophilized Glycoprotein Standard Set
Kit Contents:
Lysozyme (negative control), 2.5mg
BSA (nominal control), 2.5mg
Ovalbumin, 2.5mg
Apo-Transferrin, 2.5mg
Fetuin, 0.25mg
alpha 1-Acid Glycoprotein, 0.25mg
Kit MSDS for product # 23259 Lyophilized Glycoprotein Standard Set $183.00

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