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YOU ARE HERE:  Browse Product Catalog > Western Blotting, ELISA and Cell Imaging > Immunohistochemistry Reagents > ß-Galactosidase Precipitating Substrates  


ß-Galactosidase Precipitating Substrates 
For detection of ß-Galactosidase in immunohistochemical and clone expression applications

X-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) is a chromogenic substrate for
ß-Galactosidase. It yields a blue precipitate, is soluble in DMF, and can be used to detect vectors, plasmids, or DNA fragments that contain the ß-Galactosidase gene.

IPTG (Isopropyl-ß-D-thiogalactopyranoside) may be used to maximize the expression of genes in expression vectors. When screening a lambda bacteriophage library with an antibody, the probability of success can be increased by preventing the expression of the fusion protein until the plaques are well established. By placing a nitrocellulose filter containing the inducer IPTG onto the plate and continuing growth at 37°C, the expression of the potential ß-galactosidase-cDNA fusion protein can be induced after 3-4 hours of plaque growth. The nitrocellulose filter then is screened with antibodies. IPTG is also used in combination with X-Gal to detect active ß-galactosidase, and it is practical in applications using Bacteriophage M13 and Igt11 vectors. (1) 

References:

1. Maniatis, T., Fritsch, E. and Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual.
New York: Cold Spring Harbor Laboratory.


Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 34060 IPTG 1 G MSDS for product # 34060 IPTG $52.00
Add 34050 X-Gal 100 MG Product Instructions MSDS for product # 34050 X-Gal $77.00

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