• Incredible Sensitivity
Fifty times more sensitive than the traditional DAB method and thirty times more sensitive than other metal-intensified versions of DAB!

• Low Background, High Intensity
Get a crisp dark brown-black precipitate that’s more intense than the dull brown precipitate observed when using DAB without enhancement. And, even with the increased intensity, background is almost nonexistent!

• Only TWO Components
Simply mix the two liquid components and your working solution is ready to use.

• Six-Hour Stability
The innovative working solution is stable for more than six hours at room temperature! Other DAB substrates must be used immediately.

Substrate		Lower Detection Limit5
DAB Substrate (Method of Graham and Karnovsky3)		0.800 ng Horseradish peroxidase-conjugated streptavidin
Metal Enhanced DAB Substrate (Method of Adams4)		0.550 ng Horseradish peroxidase-conjugated streptavidin
Metal Enhanced DAB Substrate Kit (Pierce Product # 34065)		0.017 ng Horseradish peroxidase-conjugated streptavidin

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Prostatic Acid Phosphatase stained with Pierce Metal DAB and blocked with SuperBlock Blocking Buffer in TBS		Prostatic Acid Phosphatase stained by DAB (Graham and Karnovsky, 1966)

The Pierce Metal Enhanced DAB Substrate Kit Utilizes cobalt choloride and nickel chloride in a special formulation to produce a substrate with many advantages. To prepare the product, the 10X Metal Enhanced Substrate Solution is diluted with the Pierce Stable Peroxide Buffer prior to use. The special formulation of this product allows the 1X substrate to be stable for at least 6 hours at room temperature. The background color of the substrate does not increse and unlike the typical metal enhanced DAB methods, the reactivity is unchanged.^1,2 The resulting precipate is a dark brown-black that is more intense than the brown color from DAB alone. The intensity not only increases, the background is also reduced. This increases the signal-to-noise ratio, making it easier to screen tissue or membrane antigens. Along with the detection efficiency, the sensitivity is increased as determined by the lower detectability limit in a dot blot, compared to Graham and Karnovsky's DAB method and Adam's metal enhanced DAB method.

References:

1. Adams, J.C. (1977). Neuroscience 2, 141-145.
2. Adams, J.C. (1981). J. Histochem. Cytochem. 29, 775.
3. Georghegan, W.D. and Ackerman, G.A. (1977). J. Histochem. Cytochem. 25, 1187.
4. Graham, R.C. and Karnovsky, M.J. (1966). J. Histochem. Cytochem. 14, 291-302.
5. Heimberger, A.B., et al. (2003). Clin. Cancer Res. 9, 4247-4254.
6. Rodbard, D. (1978). Anal. Biochem. 90, 1-12.
7. Wanaget, J., et al. (2001). FASEB J. 15, 322-332.