Unique technology for improved assay precision and broader dynamic range.

Thermo Scientific Pierce Streptavidin and NeutrAvidin Coated Plates are available in a high binding capacity (HBC) format. Our patent-pending plate-coating technology offers a HBC Plate with a wider detection limit than our regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Highlights of Biotin-Binding High Capacity Plates:

• Unique plate-coating technology – results in high loading and binding capacity
  • NeutrAvidin* coated plates have up to twice the binding capacity of competitor's pre-coated plates
  • Streptavidin coated plates have up to five times the binding capacity of competitor's plates pre-coated plates
• Broader dynamic range – extends the quantitative range so there is less need for dilutions
• Save time – pre-blocked plates to reduce the number of assay steps
• Flexible assay formats – coated plates offered in 8-well strips to reduce waste

Comparison of Thermo Scientific NeutrAvidin High Binding Capacity (HBC) Coated Plate, NeutrAvidin Regular Binding Capacity (RBC) Coated Plates and a competing Streptavidin Coated High Binding Capacity Plate (CHC). Plates were incubated with various dilutions of biotinylated, phosphorylated peptide. After washing, the plates were incubated with mouse anti-phosphotyrosine antibody and then detected using an anti-mouse-FITC conjugate.

Comparison of Thermo Scientific Pierce Streptavidin High Binding Capacity (HBC) Coated Plate with competing high binding capacity plate. Plates were incubated with a biotinylated oligonucleotide, washed and probed with a complementary oligonucleotide labeled with fluorescein at various dilutions.

Related Products and Links:
Standard Binding Capacity Biotin-Binding Plates
High Sensitivity Biotin-Binding Plates
SuperBlock Blocking Buffer – or browse all blocking buffers
Secondary Antibody Selection Guide
ELISA Substrate Selection Guide
ELISA Accessories – microplate sealing tape, reagent reservoirs, microplates

* Thermo Scientific Streptavidin and NeutrAvidin High Binding Capacity (HBC) Coated Plates are available in large quantities for manufacturing applications.