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YOU ARE HERE:  Browse Product Catalog > Western Blotting, ELISA and Cell Imaging > ELISA Reagents and Coated Microplates > Coated Microplates > Streptavidin & NeutrAvidin Plates-High Sensitivity  


Streptavidin & NeutrAvidin Coated Plates-High Sensitivity 

Detect low-abundance targets with biotin-binding high-sensitivity microplates.

Add descriptive, keyword-rich alt tag here.Thermo Scientific Pierce High Sensitivity Streptavidin and NeutrAvidin Coated Plates enable detection of low concentrations of biotinylated antibodies and other proteins (i.e., 5 ng/ml). Whether detecting your signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.

Highlights of Biotin-Binding High Sensitivity Plates:

  • Sensitive – detect down to 5 ng/ml of biotinylated IgG
  • Specific – low background with high signal-to-noise ratios
  • Versatile – use either fluorescence, chemiluminescence or colorimetric detection
  • Robust – broad dynamic range
  • Ready to use – plates are supplied pre-blocked to save time
Small figures can be positioned with title and legend placed alongside.
Comparison of Thermo Scientific Biotin-binding Microplates. Biotinylated peptide (A), biotinylated Protein G (B) or biotinylated rabbit IgG (C) was incubated for 30 minutes in streptavidin-coated standard microplates (SBC), streptavidin-coated high binding capacity plates (HBC), or streptavidin-coated high sensitivity plates (HS). Plates were washed three times with Tris-buffered saline and incubated with a fluorescein-labeled peptide (2 µg/ml) for (A) or goat anti-rabbit IgG conjugated to horseradish peroxidase (100 ng/ml for B and C) for 30 minutes. Plates were washed and incubated with QuantaBlu* Fluorogenic Peroxidase Substrate. The fluorescent signal was measured at 430 nm.

 

Plate Characteristics for High Binding Capacity Biotin-Binding Plates.
Biotin-Binding Protein Plate Type Coat Volume Block Volume
(Blocker BSA)
Approximate Binding Capacity
NeutrAvidin
8-Well Strip
100 µl/well

200 µl/well

~3 pmol biotin/well
Streptavidin
8-Well Strip
100 µl/well
200 µl/well
~3.8 pmol biotin/well

Related Products and Links:
Standard Binding Capacity Biotin-Binding Plates
High Binding Capacity Biotin-Binding Plates
Blocker BSA Blocking Buffer – or browse all blocking buffers
Secondary Antibody Selection Guide
ELISA Substrate Selection Guide
ELISA Accessories – microplate sealing tape, reagent reservoirs, microplates

* Thermo Scientific Streptavidin and NeutrAvidin High Binding Capacity (HBC) Coated Plates are available in large quantities for manufacturing applications.


Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 15520 Streptavidin Coated Plates (High Sensitivity) , 8-well strip, clear
200 µl/well coating volume
250 µl/well blocking volume (with Blocker BSA)
5 plates MSDS for product # 15520 Streptavidin Coated Plates (High Sensitivity) , 8-well strip, clear Product Instructions $199.00
Add 15525 Streptavidin Coated Plates (High Sensitivity) , 8-well strip, black
200 µl/well coating volume
250 µl/well blocking volume (with Blocker BSA)
5 plates MSDS for product # 15525 Streptavidin Coated Plates (High Sensitivity) , 8-well strip, black Product Instructions $199.00
Add 15530 NeutrAvidin Coated Plates (High Sensitivity) , 8-well strip, clear
200 µl/well coating volume
250 µl/well blocking volume (with Blocker BSA)
5 plates MSDS for product # 15530 NeutrAvidin Coated Plates (High Sensitivity) , 8-well strip, clear Product Instructions $199.00
Add 15535 NeutrAvidin Coated Plates (High Sensitivity) , 8-well strip, black
200 µl/well coating volume
250 µl/well blocking volume (with Blocker BSA)
5 plates MSDS for product # 15535 NeutrAvidin Coated Plates (High Sensitivity) , 8-well strip, black Product Instructions $199.00

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