Boost plate-based assay performance by binding more His-tagged protein.

Thermo Scientific Pierce Copper Coated Plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metals such as copper. These copper-coated plates are available as clear, white or black plates that can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Pierce Copper Coated Plates have High Binding Capacity (HBC) because they are coating using an exclusive process that increases the amount of histidine-tagged protein that will bind to the plate surface. Although copper is less discriminating in its binding specificity than Nickel, the higher binding capacity of these plates is ideal for high throughput screening applications that need improved sensitivity and greater dynamic range.

Highlights:

• Quantitate previously undetectable histidine-tagged proteins
• Wider dynamic range with four-fold greater capacity than regular nickel chelate-coated plate
• Copper chelate provides greater binding capacity than nickel
• The detection limit is 0.1 pg of polyhistidine tagged fusion protein per well
• Pre-blocked for the ultimate convenience

Binding performance of nickel vs. copper coated plates. Comparison of Thermo Scientific Pierce Nickel Coated Plates to Pierce Copper Coated High Binding Capacity (HBC) Plates. Performance was evaluated comparing the amount of fluorescent signal produced by captured His-tagged fluorescent protein.

Note: Copper coated plates are for use with purified His-tagged proteins. Typical cell lysates contain non-his-tagged proteins that may bind to copper, which can reduce binding capacity and signal-to-noise ratios. For cell lysates, use Thermo Scientific Pierce Nickel Coated Plates.