Bind recombinantly expressed GST-fusion proteins for plate-based analysis.

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well polystyrene microplates whose wells have been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These glutathione coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.

Highlights:

• Selective – specifically binds fusion proteins containing glutathione S-transferase
• Capacity – binds approx. 10ng GST protein per well
• Versatile – ready-to-bind plate format is useful for protein interaction studies
• Compatible – detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
• Ready to use – Pre-blocked with BSA and SuperBlock Blocking Buffer to reduce nonspecific binding
• Three formats – available as clear, white or black 96-well microplates

Binding of GST to Thermo Scientific Pierce Glutathione Coated Plate.

References:

1. Pullen, S.S., et al. (1999). J. Biol. Chem. 274, 14246-14254.
2. McKevitt, M., et al. (2003). Genome Res. 13, 1665-1674.
3. Yarwood, S.J., et al. (1999). J. Biol. Chem. 274, 14909-14917.

Related Products:

Pierce Glutathione Agarose and GST Purification Kits
Anti-GST Antibody Coated Plates
B-PER Bacterial Protein Extraction Reagents