The specific binding affinity for biotin with nonspecific interactions blocked.

Thermo Scientific Pierce Streptavidin and NeutrAvidin Coated Plates are available in 96-well and 384-well plate formats and pre-blocked with BSA or SuperBlock Blocking Buffers. These Pierce Biotin-Binding Plates are available in clear, white and black plates can be used with colorimetric, chemiluminescent and fluorescent detection methods, respectively.

About Pierce Biotin-Biotin Binding Plates

Streptavidin and NeutrAvidin Protein are not glycosylated which prevents nonspecific lectin-binding observed with avidin coated plates. Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin has an isoelectric point of 5-6, resulting in low nonspecific interactions. NeutrAvidin Protein has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has an additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells. NeutrAvidin Protein has the lowest nonspecific binding among the known biotin-binding proteins.

Highlights of Biotin-Binding Plates:

• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Additional Highlights of NeutrAvidin Coated Plates:

• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Related Products and Links:
High Binding Capacity Biotin-Binding Plates
High Sensitivity Biotin-Binding Plates
SuperBlock Blocking Buffer – or browse all blocking buffers
Secondary Antibody Selection Guide
ELISA Substrate Selection Guide
ELISA Accessories – microplate sealing tape, reagent reservoirs, microplates

* Thermo Scientific Streptavidin and NeutrAvidin High Binding Capacity (HBC) Coated Plates are available in large quantities for manufacturing applications.