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ABC Staining Kits  |
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Systems that offer highly sensitive and rapid detection.
| ImmunoPure ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Highly diluted primary antibodies can be used with Pierce’s ImmunoPure Staining Kits, producing comparable stain intensity to other methods that require higher concentrations of antibody.
Two types of enzymatic ABC staining kits are available: alkaline phosphatase and horseradish peroxidase. The kits can come with, or without (Standard Kit) a biotinylated secondary antibody. Kits with a secondary antibody are selected by the species of primary antibody to be used. For example, if the primary antibody (IgG) is produced in mice, the kit selected to detect this antibody should be the ImmunoPure ABC Mouse IgG Kit. All you need to create a sensitive detection system is your specific primary antibody, an ImmunoPure ABC Kit with biotinylated antibody, and an enzyme substrate.
A Standard Kit includes only the avidin and biotinylated enzyme. It is useful if an ABC Kit is not available for your specific species of primary antibody – or if the specificity of the antibody in the kit is not appropriate for the application. The biotinylated antibody for your specific application can then be purchased separately, along with blocking serum, and used with a standard kit.
The Ultra-Sensitive ABC Peroxidase Staining Kits are more sensitive than the ABC Peroxidase Staining Kits, without exhibiting increased background staining. These kits supply the extra sensitivity needed for localizing antigens present in very small quantities. An expensive primary antibody may be diluted approximately five-fold higher than with the ABC Peroxidase Kit – while producing equal staining intensity.2
ImmunoPure ABC Kit Contents:
- 3 ml of the appropriate blocking serum
- 1 ml of the biotinylated affinity-purified secondary antibody
- 2 ml each of the avidin and biotinylated enzyme reagents
Suggested Substrates for Use with Horseradish Peroxidase Kits
ELISA:
TMB Substrate Kit (#34021)
1-Step Turbo TMB (#34022)
1-Step ABTS (#37615)
Western Blotting:
1-Step Chloronaphthol (#34012)
1-Step TMB-Blotting (#34018)
Histochemical:
Metal Enhanced DAB Substrate Kit (#34065)
Chemiluminescent:
SuperSignal West Pico Substrate (#34080)
SuperSignal West Dura Substrate (#34075)
Suggested Substrates for Use with Alkaline Phosphatase Kits
ELISA:
Phosphatase Substrate Kit (contains PNPP)(#37620)
Histochemical:
1-Step NBT/BCIP (#34042)
NBT/BCIP Substrate Kit (#34041)
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References
- Savage,M.D., Mattson,G.Desai,S.,Nielander,G.W.,Morgensen,S.and Conklin,E.J.(1992). Avidin-Biotin Chemistry:A Handbook.Rockford,Illinois:Pierce Chemical Company(Product #150555)
- Bayer,E.A,Ben-Hur,H.,Wilcheck,M.(1988).Biocytin-hydrazide:a selective label for sialic acids,galactose, and other sugars in glycoconjugates using avidin/biotin technology,Anal.Biochem. 170,271-281
Ordering Information
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 Certificate of Analysis  Product Instructions  MSDS |
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Product # |
Description |
Pkg. Size |
Files |
Price |
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32020 |
ABC Standard Peroxidase Staining Kit
| Kit contains: | | Reagent A: Avidin | 2 ml | | Reagent B: Biotinylated HRP | 2 ml |
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Kit |
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$260.00 |
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32050 |
ABC Ultra-Sensitive Peroxidase Staining Kit
| Kit contains: | | Reagent A: Avidin | 2 ml | | Reagent B: Biotinylated HRP | 2 ml |
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Kit |
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$343.00 |
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Add
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32052 |
Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit
| Kit contains: | | Reagent A: Avidin | 2 ml | | Reagent B: Biotinylated HRP | 2 ml | | Blocking Buffer, contains normal horse serum | 3 ml | | Biotinylated Anti-Mouse IgG Antibody, 1.5 mg/ml | 1 ml |
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Kit |
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$487.00 |
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Add
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32054 |
Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit
| Kit contains: | | Reagent A: Avidin | 2 ml | | Reagent B: Biotinylated HRP | 2 ml | | Blocking Buffer, contains normal goat serum | 3 ml | | Biotinylated Anti-Rabbit IgG Antibody, 1.5 mg/ml | 1 ml |
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Kit |
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$475.00 |
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