The high-performance, lower-cost alternative to streptavidin resins.

Thermo Scientific Immobilized NeutrAvidin Products are superior alternatives to avidin and streptavidin agarose and acrylamide beaded affinity resins. NeutrAvidin Protein is a modified avidin derivative that combines several key features to provide a biotin-binding protein with exceptionally low nonspecific binding properties. Native avidin is heavily glycosylated, resulting in its high pI and nonspecific binding properties. By contrast NeutrAvidin Protein contains no carbohydrate and has a near-neutral isoelectric point (6.3), features that provide for very low nonspecific binding and broader utility in a variety of applications.

NeutrAvidin Resins are prepared by covalently coupling NeutrAvidin Protein to the beaded agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in NeutAvidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies. To determine the best reagent to biotinylate a protein or other biomolecule, visit the Biotinylation Reagent Selection Guide.

Highlights:

• Low nonspecific binding – Unlike avidin, NeutrAvidin Protein has no carbohydrates, eliminating nonspecific binding problems caused by sugars; unlike streptavidin, NeutrAvidin Protein has no Arg-Tyr-Asp sequence, which can be a source of binding to cell surface molecules.
• Proven coupling chemistries – The methods used to prepare these resins are superior to typical cyanogen bromide (CNBr) immobilization, providing high functionality, low nonspecific binding and excellent leach-resistance.
• Versatile resins – Agarose (polysaccharide-based) and UltraLink Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods.
• Available in multiple formats – Choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available).
• Superior performance – High Capacity Resin has higher binding capacity than best alternative supplier streptavidin products, allowing use of small amounts of resin for experiments.

Related Products:

Streptavidin Agarose and UltraLink Resins
Avidin Agarose Resin
Pierce Monomeric Avidin Resins and Kit
Purified NeutrAvidin and NeutrAvidin Conjugates
NeutrAvidin Coated Plates

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