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Jacalin is an a -D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein of approximately 40,000 MW composed of four identical subunits. Jacalin immobilized on supports such as agarose is useful for the purification of human serum or secretory IgA1. IgA can be separated from human IgG and IgM in human serum or colostrums using Immobilized Jacalin. This support is also useful for removing contaminating IgA from IgG samples. Immobilized Jacalin is preferable to protein A because protein A will bind some IgA along with IgG and is not effective for IgA removal.
Highlights of Immobilized Jacalin
- Ideal for preparing Human IgA that is free of contaminating IgG
- Found to bind Human IgA1, but not Human IgA2 – useful for separating the two subclasses
| Properties of Immobilized Jacalin |
| Support: |
6% crosslinked beaded agarose |
| Loading: |
~ 4.5 mg Jacalin per ml of gel. |
| Binding capacity: |
1-3 mg human IgA per ml of gel |
| Storage: |
Store at 4°C. Do not freeze. |
Related Products
Products for Antibody Purification
References
- Kumar, G.S., Appukuttan, P.S. and Basu, D. (1982). J. Biosci. 4, 257-261.
- Roque-Barriera, M.C. and Campos-Neto, A. (1985). J. Immunol. 134, 1740-1743.
- Mestecky, J., Julhavy, R. and Kraus, F.W. (1971). J. Immunol. 107, 605.
- Van Kamp, G.J. (1979). J. Immunol. Meth. 27, 301.
- Kondoh, H., et al. (1986). J. Immunol. Meth. 88, 171-173.
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