High binding, low leaching supports
for antibody purification and immunoprecipitation.
Thermo Scientific Pierce Protein A/G Resins are effective for affinity purification of IgG from serum and other fluids of many species. Protein A/G is especially suited for polyclonal antibody purification because it binds the broadest range of antibody species and subclasses. Our Protein A/G Agarose and UltraLink Resins use recombinant Protein A/G immobilized using chemistries that are leak-resistant, providing high-capacity affinity supports with minimal nonspecific binding. These agarose and polyacrylamide supports can be regenerated and reused multiple times when stored properly. |
|
Protein A/G Beads - Quick Reference
|
| Protein A/G |
Recombinant protein expressed in E. coli (50.5 kDa; six IgG-binding sites) |
Specificity
(see details) |
Best for polyclonal IgG from many species; poor for individual subclasses that have highest affinity for Protein A or Protein G. |
Supports
Offered |
Crosslinked 6% beaded agarose
UltraLink Biosupport
|
Package
Formats |
Resin slurries (3 sizes)
Chromatography Cartridges (2 sizes)
Packed Spin Columns (3 sizes)
NAb IgG Purification Kits (2 sizes)
|
| Storage |
4°C, do not freeze |
|
Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. It is a gene fusion product expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons (40-45 kDa by SDS-PAGE). Protein A/G is not as pH dependent as Protein A, but otherwise has the additive properties of Protein A and G.
Protein A/G binds to all human IgG subclasses, making it the ideal choice for purification of polyclonal or monoclonal IgG antibodies whose subclass identities have not been determined. In addition, it binds to IgA, IgE, IgM and (to a lesser extent) IgD. Protein A/G also binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM or serum albumin [1]. This makes Protein A/G an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and murine serum albumin. Individual subclasses of mouse monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G [2].
Protein A/G Resin Highlights:
- Single support to provide all the benefits of Protein A and Protein G
- Binds to a wider range of antibodies than Protein A or Protein G beads
- Efficient and leach-resistant immobilization chemistries provide for high capacity and purity for antibody purification
- Available in regular and high ("Plus") binding-capacity formats
- Offered as resin slurries, chromatography cartridges, NAb Spin Columns and Purification Kits
(Learn more about Pierce Chromatography Cartridges or about NAb Spin Columns.)
Choose between Protein A, Protein G, Protein L:
Related Products:
References:
- Sikkema, J.W.D. (1989) Amer. Biotech. Lab, 7, 42.
- Eliasson, M., et al. (1988) J. Biol. Chem. 263, 4323-4327.
- Alekseev, O.M., et al. (2005) J. Biol. Chem. 280, 2904-2911.
- Chackerian, B., et al. (2004) J. Virol. 78, 4037-4047.
- Ecsedy, J.A., et al. (2003) Mol. Cell. Biol. 23, 950-960.
|
Certificate of Analysis
Product Instructions
MSDS