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Protein A Agarose and UltraLink Resins  |
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High binding, low leaching supports
for antibody purification and immunoprecipitation.
Thermo Scientific Pierce Protein A Agarose and UltraLink Resins are effective for affinity purification of IgG from serum and other fluids of many species. Protein A is especially suited for polyclonal antibody purification from rabbits. Our Protein A Agarose and UltraLink Resins use the native form of Protein A immobilized using chemistries that are leak-resistant, providing high-capacity affinity supports with minimal nonspecific binding. These agarose and polyacrylamide supports can be regenerated and reused multiple times when stored properly. |
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Protein A Beads - Quick Reference
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| Protein A |
Native protein purified from
Staphylococcus aureus
(46.7 kDa; four IgG-binding sites) |
Specificity
(see details) |
Best for polyclonal IgG from rabbit, pig, dog, cat serum; poor for Mouse IgG1, human IgG3, rat, goat, cow |
Supports
Offered |
Crosslinked 6% beaded agarose
UltraLink Biosupport
Trisacryl GF-2000 Resin
Magnetic particles (1-4 µm)
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Package
Formats |
Resin slurries (3 sizes)
Chromatography Cartridges (2 sizes)
Packed Spin Columns (3 sizes)
NAb IgG Purification Kits (2 sizes)
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| Storage |
4°C, do not freeze |
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Protein A is a cell wall component produced by several strains of Staphylococcus aureus. It consists of a single polypeptide chain (MW 46,762) and contains little or no carbohydrate. Protein A binds specifically to the Fc region of immunoglobulin molecules, especially IgG. It has four high-affinity (Ka = 108/mole) binding sites that are capable of interacting with the Fc region of IgGs of several species (only two sites can bind at a time). The molecule is heat-stable and retains its native conformation even after exposure to denaturing reagents such as 4 M urea, 4 M thiocyanate and 6 M guanidine hydrochloride.
Protein A Resin Highlights:
- Efficient and leach-resistant immobilization chemistries provide for high capacity and purity for antibody purification
- Binds to a wide range of antibodies - especially good for purification of rabbit IgG
- Less expensive than Protein G agarose
- Available in regular and high ("Plus") binding-capacity formats
- Offered as resin slurries, chromatography cartridges, NAb Spin Columns and Purification Kits
(Learn more about Pierce Chromatography Cartridges or about NAb Spin Columns.)
Choose between Protein A, Protein G, Protein L:
Related Products:
References:
- Sjoquist, J., et al. (1972) Eur. J. Biochem.29, 572-578.
- Hjelm, H., et al. (1975) Eur. J. Biochem.57, 395-403.
- Sjoholm, I., et al. (1975) Eur. J. Biochem.51, 55-6.
- Wang, B., et al. (1999) Proc. Nat. Acad. Sci. USA.96, 1627-1632.
- Higashi, I. , et al. (2000) Clin. Chem. 46, 297-299.
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Certificate of Analysis
Product Instructions
MSDS