Advanced
Part of Thermo Fisher Scientific
Thermo Scientific - Pierce Protein Research Products  

Document Center

  Certificate of Analysis
  Product Instructions
  MSDS
  Request a Catalog

User Profile

Email Address:
Password:
 
  Forgot Password
  Register
  Why Register?

Shopping Cart

 Cart is Empty
 Quick Order
  Ordering Information
Shipping & Handling
Returns Info
Security Info
Terms & Conditions



Email Sign-up  Email Sign-up

Subscribe to our RSS feed!  RSS

Follow us on Facebook  Follow Us


 




 Protein A Agarose and UltraLink Resins 

High binding, low leaching supports
for antibody purification and immunoprecipitation.

Thermo Scientific Pierce Protein A Agarose and UltraLink Resins are effective for affinity purification of IgG from serum and other fluids of many species. Protein A is especially suited for polyclonal antibody purification from rabbits. Our Protein A Agarose and UltraLink Resins use the native form of Protein A immobilized using chemistries that are leak-resistant, providing high-capacity affinity supports with minimal nonspecific binding. These agarose and polyacrylamide supports can be regenerated and reused multiple times when stored properly.

Protein A Beads - Quick Reference

Protein A Native protein purified from
Staphylococcus aureus
(46.7 kDa; four IgG-binding sites)
Specificity
(see details)
Best for polyclonal IgG from rabbit, pig, dog, cat serum; poor for Mouse IgG1, human IgG3, rat, goat, cow
Supports
Offered

Crosslinked 6% beaded agarose
UltraLink Biosupport
Trisacryl GF-2000 Resin
Magnetic particles (1-4 µm)

Package
Formats

Resin slurries (3 sizes)
Chromatography Cartridges (2 sizes)
Packed Spin Columns (3 sizes)
NAb IgG Purification Kits (2 sizes)

Storage 4°C, do not freeze

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. It consists of a single polypeptide chain (MW 46,762) and contains little or no carbohydrate. Protein A binds specifically to the Fc region of immunoglobulin molecules, especially IgG. It has four high-affinity (Ka = 108/mole) binding sites that are capable of interacting with the Fc region of IgGs of several species (only two sites can bind at a time). The molecule is heat-stable and retains its native conformation even after exposure to denaturing reagents such as 4 M urea, 4 M thiocyanate and 6 M guanidine hydrochloride.

Protein A Resin Highlights:

  • Efficient and leach-resistant immobilization chemistries provide for high capacity and purity for antibody purification
  • Binds to a wide range of antibodies - especially good for purification of rabbit IgG
  • Less expensive than Protein G agarose
  • Available in regular and high ("Plus") binding-capacity formats
  • Offered as resin slurries, chromatography cartridges, NAb Spin Columns and Purification Kits
    (Learn more about Pierce Chromatography Cartridges or about NAb Spin Columns.)

Choose between Protein A, Protein G, Protein L:

Related Products:

References:

  1. Sjoquist, J., et al. (1972) Eur. J. Biochem.29, 572-578.
  2. Hjelm, H., et al. (1975) Eur. J. Biochem.57, 395-403.
  3. Sjoholm, I., et al. (1975) Eur. J. Biochem.51, 55-6.
  4. Wang, B., et al. (1999) Proc. Nat. Acad. Sci. USA.96, 1627-1632.
  5. Higashi, I. , et al. (2000) Clin. Chem. 46, 297-299.


Ordering Information
Bulk quantities: InquireBulk quantities: Inquire    Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Product # Description Pkg. Size Files Price
89924 Pierce Protein A Chromatography Cartridges, 1mL
Learn more about Pierce Chromatography Cartridges.
2 cartridges Product Instructions MSDS for product # 89924 Pierce Protein A Chromatography Cartridges, 1mL $160.00

89925 Pierce Protein A Chromatography Cartridges, 5mL
Learn more about Pierce Chromatography Cartridges.
1 cartridge Product Instructions MSDS for product # 89925 Pierce Protein A Chromatography Cartridges, 5mL $320.00

20333 Pierce Protein A Agarose

Support: Crosslinked 6% beaded agarose
Capacity: > 12-19mg human IgG/mL of resin

5mL Product Instructions MSDS for product # 20333 Pierce Protein A Agarose $325.00

20334 Pierce Protein A Agarose

Support: Crosslinked 6% beaded agarose
Capacity: > 12-19mg human IgG/mL of resin

25mL Product Instructions MSDS for product # 20334 Pierce Protein A Agarose $988.00

20338 Protein A Trisacryl Resin

Support: Trisacryl GF-2000
Capacity: > 15mg human IgG/mL of resin

5mL Product Instructions MSDS for product # 20338 Protein A Trisacryl Resin $341.00

20356 Pierce Protein A Columns, 1mL 5 columns Product Instructions MSDS for product # 20356 Pierce Protein A Columns, 1mL $369.00

44667 Protein A IgG Purification Kit, 1mL
Contains everything needed to isolate IgG from mouse ascites or other serum.
Kit contents:
Protein A Columns, 1mL, 5 columns
Protein A IgG Binding Buffer, 1L
IgG Elution Buffer, 500mL
Excellulose Desalting Columns, 5mL, 5 columns
5-column kit Product Instructions MSDS for product # 44667 Protein A IgG Purification Kit, 1mL $500.00

22810 Pierce Protein A Plus Agarose

Support: Crosslinked 6% beaded agarose
Capacity: > 34mg human IgG/mL of resin; 16-17mg mouse IgG/mL of resin

1mL Product Instructions MSDS for product # 22810 Pierce Protein A Plus Agarose $105.00

22811 Pierce Protein A Plus Agarose

Support: Crosslinked 6% beaded agarose
Capacity: > 34mg human IgG/mL of resin; 16-17mg mouse IgG/mL of resin

5mL Product Instructions MSDS for product # 22811 Pierce Protein A Plus Agarose $380.00

22812 Pierce Protein A Plus Agarose

Support: Crosslinked 6% beaded agarose
Capacity: > 34mg human IgG/mL of resin; 16-17mg mouse IgG/mL of resin

25mL Product Instructions MSDS for product # 22812 Pierce Protein A Plus Agarose $1,273.00

89952 NAb Protein A Plus Spin Columns, 0.2mL 10 columns Product Instructions MSDS for product # 89952 NAb Protein A Plus Spin Columns, 0.2mL $215.00

89956 NAb Protein A Plus Spin Columns, 1mL
Replaces product: 22814
5 columns Product Instructions MSDS for product # 89956 NAb Protein A Plus Spin Columns, 1mL $411.00

89960 NAb Protein A Plus Spin Column, 5mL 1 column Product Instructions MSDS for product # 89960 NAb Protein A Plus Spin Column, 5mL $381.00

89948 NAb Protein A Plus Spin Kit, 0.2mL
Kit contents:
NAb Protein A Plus Spin Columns, 0.2mL, 10 columns
Microcentrifuge Tubes, 2mL, 80 tubes
PBS Pack (makes 500mL), 1 pack
IgG Elution Buffer (pH 2.8), 50mL
Neutralization Buffer, 12mL
10-column kit Product Instructions MSDS for product # 89948 NAb Protein A Plus Spin Kit, 0.2mL $263.00

89978 NAb Protein A Plus Spin Kit, 1mL
Kit contents:
NAb Protein A Plus Spin Columns, 1mL, 2 columns
PBS Pack (makes 500mL), 1 pack
IgG Elution Buffer (pH 2.8), 240mL
Neutralization Buffer, 12mL
2-column kit MSDS for product # 89978 NAb Protein A Plus Spin Kit, 1mL Product Instructions $253.00

53139 Protein A UltraLink Resin

Support: UltraLink Biosupport
Capacity: > 16mg human IgG/mL of resin

5mL Product Instructions MSDS for product # 53139 Protein A UltraLink Resin $325.00

53142 Protein A Plus UltraLink Resin

Support: UltraLink Biosupport
Capacity: > 30mg human IgG/mL of resin

5mL Product Instructions MSDS for product # 53142 Protein A Plus UltraLink Resin $382.00

21181 Pierce Protein A 5mg Product Instructions MSDS for product # 21181 Pierce Protein A $128.00


Learn more about other Thermo Scientific Life Science Research Products
Biopolymers Cell Culture High Content Screening  
Nucleic Acid Technologies PCR Reagents & Plastics RNAi Technologies  European Sales

 © 2010 Thermo Fisher Scientific Inc.

PO Box 117, Rockford, IL 61105 USA
1-800-874-3723  or  815-968-0747

Trademarks Privacy Statement