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Protein A Agarose and UltraLink Resins |
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High binding, low leaching supports
for antibody purification and immunoprecipitation.
Thermo Scientific Pierce Protein A Agarose and UltraLink Resins are effective for affinity purification of IgG from serum and other fluids of many species. Protein A is especially suited for polyclonal antibody purification from rabbits. Our Protein A Agarose and UltraLink Resins use the native form of Protein A immobilized using chemistries that are leak-resistant, providing high-capacity affinity supports with minimal nonspecific binding. These agarose and polyacrylamide supports can be regenerated and reused multiple times when stored properly. |
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Protein A Beads - Quick Reference
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| Protein A |
Native protein purified from
Staphylococcus aureus
(46.7 kDa; four IgG-binding sites) |
Specificity
(see details) |
Best for polyclonal IgG from rabbit, pig, dog, cat serum; poor for Mouse IgG1, human IgG3, rat, goat, cow |
Supports
Offered |
Crosslinked 6% beaded agarose
UltraLink Biosupport
Trisacryl GF-2000 Resin
Magnetic particles (1-4 µm)
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Package
Formats |
Resin slurries (3 sizes)
Chromatography Cartridges (2 sizes)
Packed Spin Columns (3 sizes)
NAb IgG Purification Kits (2 sizes)
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| Storage |
4°C, do not freeze |
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Protein A is a cell wall component produced by several strains of Staphylococcus aureus. It consists of a single polypeptide chain (MW 46,762) and contains little or no carbohydrate. Protein A binds specifically to the Fc region of immunoglobulin molecules, especially IgG. It has four high-affinity (Ka = 108/mole) binding sites that are capable of interacting with the Fc region of IgGs of several species (only two sites can bind at a time). The molecule is heat-stable and retains its native conformation even after exposure to denaturing reagents such as 4 M urea, 4 M thiocyanate and 6 M guanidine hydrochloride.
Protein A Resin Highlights:
- Efficient and leach-resistant immobilization chemistries provide for high capacity and purity for antibody purification
- Binds to a wide range of antibodies - especially good for purification of rabbit IgG
- Less expensive than Protein G agarose
- Available in regular and high ("Plus") binding-capacity formats
- Offered as resin slurries, chromatography cartridges, NAb Spin Columns and Purification Kits
(Learn more about Pierce Chromatography Cartridges or about NAb Spin Columns.)
Choose between Protein A, Protein G, Protein L:
Related Products:
References:
- Sjoquist, J., et al. (1972) Eur. J. Biochem.29, 572-578.
- Hjelm, H., et al. (1975) Eur. J. Biochem.57, 395-403.
- Sjoholm, I., et al. (1975) Eur. J. Biochem.51, 55-6.
- Wang, B., et al. (1999) Proc. Nat. Acad. Sci. USA.96, 1627-1632.
- Higashi, I. , et al. (2000) Clin. Chem. 46, 297-299.
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Ordering Information
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 Bulk quantities: Inquire  Certificate
of Analysis  Product
Instructions  MSDS |
| Product # |
Description |
Pkg. Size |
Files |
Price |
| 89924 |
Pierce Protein A Chromatography Cartridges, 1mL
Learn more about Pierce Chromatography Cartridges. |
2 cartridges |
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$160.00
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| 89925 |
Pierce Protein A Chromatography Cartridges, 5mL
Learn more about Pierce Chromatography Cartridges. |
1 cartridge |
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$320.00
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| 20333 |
Pierce Protein A Agarose
| Support: Crosslinked 6% beaded agarose
Capacity: > 12-19mg human IgG/mL of resin
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5mL |
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$325.00
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| 20334 |
Pierce Protein A Agarose
| Support: Crosslinked 6% beaded agarose
Capacity: > 12-19mg human IgG/mL of resin
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25mL |
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$988.00
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| 20338 |
Protein A Trisacryl Resin
| Support: Trisacryl GF-2000
Capacity: > 15mg human IgG/mL of resin
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5mL |
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$341.00
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| 20356 |
Pierce Protein A Columns, 1mL |
5 columns |
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$369.00
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| 44667 |
Protein A IgG Purification Kit, 1mL
Contains everything needed to isolate IgG from mouse ascites or other serum.
Kit contents:
Protein A Columns, 1mL, 5 columns
Protein A IgG Binding Buffer, 1L
IgG Elution Buffer, 500mL
Excellulose Desalting Columns, 5mL, 5 columns
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5-column kit |
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$500.00
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| 22810 |
Pierce Protein A Plus Agarose
| Support: Crosslinked 6% beaded agarose
Capacity: > 34mg human IgG/mL of resin; 16-17mg mouse IgG/mL of resin
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1mL |
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$105.00
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| 22811 |
Pierce Protein A Plus Agarose
| Support: Crosslinked 6% beaded agarose
Capacity: > 34mg human IgG/mL of resin; 16-17mg mouse IgG/mL of resin
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5mL |
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$380.00
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| 22812 |
Pierce Protein A Plus Agarose
| Support: Crosslinked 6% beaded agarose
Capacity: > 34mg human IgG/mL of resin; 16-17mg mouse IgG/mL of resin
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25mL |
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$1,273.00
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| 89952 |
NAb Protein A Plus Spin Columns, 0.2mL |
10 columns |
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$215.00
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| 89956 |
NAb Protein A Plus Spin Columns, 1mL
Replaces product: 22814 |
5 columns |
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$411.00
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| 89960 |
NAb Protein A Plus Spin Column, 5mL |
1 column |
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$381.00
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| 89948 |
NAb Protein A Plus Spin Kit, 0.2mL
Kit contents:
NAb Protein A Plus Spin Columns, 0.2mL, 10 columns
Microcentrifuge Tubes, 2mL, 80 tubes
PBS Pack (makes 500mL), 1 pack
IgG Elution Buffer (pH 2.8), 50mL
Neutralization Buffer, 12mL
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10-column kit |
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$263.00
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| 89978 |
NAb Protein A Plus Spin Kit, 1mL
Kit contents:
NAb Protein A Plus Spin Columns, 1mL, 2 columns
PBS Pack (makes 500mL), 1 pack
IgG Elution Buffer (pH 2.8), 240mL
Neutralization Buffer, 12mL
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2-column kit |
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$253.00
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| 53139 |
Protein A UltraLink Resin
| Support: UltraLink Biosupport
Capacity: > 16mg human IgG/mL of resin
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5mL |
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$325.00
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| 53142 |
Protein A Plus UltraLink Resin
| Support: UltraLink Biosupport
Capacity: > 30mg human IgG/mL of resin
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5mL |
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$382.00
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| 21181 |
Pierce Protein A |
5mg |
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$128.00
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