Strong, dialyzable ionic detergent for formulation of RIPA buffers and other applications requiring solubilization of protein or disruption of protein interactions.

Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions. Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer) but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing nonspecific binding in affinity chromatography and a cell culture media supplement.

Highlights of Sodium Deoxycholate:

• Popular anionic, bile-acid detergent for many laboratory uses
• Effective in disrupting and dissociating many types of protein interaction
• Can be removed from solution by dialysis
• Useful for elution or regeneration of certain kinds of affinity columns
• High-purity compound with low UV absorptivity

Properties of Sodium deoxycholic acid:

• Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt
• Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt
• Molecular Weight: 414.6g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 5 (average)
• Micelle Molecular Weight: 2000g (average)
• Critical Micelle Concentration (CMC): 2 to 6mM (0.083 to 0.249%, w/v)
• Cloud Point: Unknown
• Dialyzable: Yes

Product Details:

Sodium Deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.

The effectiveness of a detergent in any application is dependent on the detergents concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2mM, cholate will form micelles having MW ~2000. The small size of these micelle allows them to be easily remove by dialysis or gel filtration if needed. Note: removal of a detergent from a protein solution my result in protein precipitation and/or aggregation.

Specifications for Sodium Deoxycholate (Part No. 89904, 89905):

• Formula: C₂₄H₃₉O₄Na
• Molecular Weight: 414.6g
• Purity (by HPLC): ≥98%
• Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06
• pH (1% Solution): 5 to 9
• Solubility (in water at 20°C): ≥5%

Related Products and Resources:

Detergent Sampler Pack
Browse all Thermo Scientific Pierce Detergents
Protein Methods Library: Detergents for Cell Lysis