Anti-rabbit IgG and other secondary antibodies
Affinity-purified polyclonal antibodies for a variety of applications.

The Thermo Scientific Pierce Secondary Antibodies featured here are affinity-purified polyclonal antibodies with well-characterized specificity for rabbit immunoglobulin (IgG). Each antibody is affinity-purified using its target antigen (i.e., the target rabbit primary antibody and fragment) coupled to an agarose support. Affinity purification eliminates nonspecific antibodies, resulting in high specificity and low background. The purification process involves an elution procedure, that yields antibodies with high avidity. Conjugated antibodies are affinity-purified before being labeled and contain bovine serum albumin as a stabilizer.

Selected secondary antibodies have been further purified to minimize cross-reactivity to serum proteins of other species. This purification is accomplished by adsorbing the antigen-purified antibody to an affinity column containing immobilized serum proteins of selected species. This additional processing minimizes antibody cross-reactivity to the serum of proteins of the species indicated by the code "min x Sp Sr Prot," where the cross-absobed species are indicated by the accompanying abbreviation key.

Related Product Groups and Selection Guides

• DyLight Antibody Conjugates – fluorescent anti-mouse and anti-rabbit secondary antibodies
• High Sensitivity and Poly-HRP Conjugates – maximizes signal in ELISA, Western blot and IHC
• Stabilized HRP Conjugates – pre-diluted (10 µg/ml), stable solutions for maximum convenience
• Guardian Peroxidase Stabilizer – store reconstituted conjugates at 4°C as 1:1,000 stock solutions
• Secondary Antibody Selection Guide – find antibodies by host and species specificity and label

The items below are arranged in the following order:

1. Unlabeled antibodies
2. Alkaline phosphatase (AP) conjugates
3. Horseradish peroxidase (HRP) conjugates
4. Fluorescein and other fluorescent conjugates
5. Biotin conjugates