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Zeba Spin Desalting Columns and Devices, 7K MWCO


Quickly remove salts and small molecules from protein samples.

Zeba Spin Desalting Columns, Desalting Plates, Desalting Cartridges.

Thermo Scientific Zeba Spin Desalting Columns, Plates and Cartridges (7K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 2µL to 4mL samples.

Zeba Desalting Columns, Plates and Cartridges are polypropylene devices containing high-performance Zeba Desalting Resin, offering rapid and exceptional protein desalting with high recovery of proteins larger than 7000 daltons. Available in 5 different spin columns sizes, 96-well filter spin plates and cartridges, Zeba Desalting Devices provide trouble-free desalting and buffer exchange for sample volumes ranging from 2µL to 4mL. The easy-to-use spin column format dramatically improves results over standard drip-column methodologies and the 96-well spin plate format enables more high-throughput processing. The Zeba Desalting Cartridges are compatible with syringe processing or will attach to popular automated liquid-chromatography systems.

Highlights:

  • High recovery – low-binding resin maximizes protein recovery
  • Fast – no fraction screening or waiting for protein to emerge by gravity-flow
  • Easy-to-use – no cumbersome column preparation or equilibration
  • Flexible – available in spin columns, filter spin plates and cartridges for a range of needs
  • Economical – great performance at lower cost than other commercially available cartridges

Product Details:

Video of Zeba Spin Desalting Columns in action!

Zeba Spin Desalting Columns, 7K MWCO contain a proprietary high-performance desalting resin with exceptional desalting and protein-recovery characteristics compared to other commercially available resins. Even very dilute (25µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (< 1000 MW) and good recovery of proteins and other macromolecules (> 7000 MW). Zeba Spin Desalting Columns can be used to process samples from 2 to 4000µL.

Zeba Spin Desalting Columns for high recovery and minimal dilution

Better performance with Zeba Spin Desalting Columns. Thermo Scientific Zeba Columns provide higher protein recovery and less sample dilution over a wider range of sample concentrations and volume compared to alternative products. Zeba Spin Desalting Columns (7K MWCO, 10mL) and Disposable PD-10 Desalting Columns (#17-0851-01, GE Healthcare) were used to desalt 1.5, 2.5 and 3.5mL samples of bovine serum albumin (BSA) at concentrations of 0.04, 0.2, and 1mg/mL. Desalting was performed according to each manufacturer’s recommended protocols for either spin (centrifuge) or drip (gravity) procedures. Three sets of columns were equilibrated in final buffer and then loaded with 1.5, 2.5, and 3.5mL samples. For each electrophoresis gel, an aliquot of starting sample equal to 1µg of BSA was loaded in Lane 1 as the Load Control; all other desalted samples were loaded in the gel at the same volume as the Load Control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting.

The Zeba 96-well Spin Desalting Plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100µL) in 5 minutes.

Protein recovery and desalting efficiency. Protein samples (1mg/mL) were prepared in 1.0M NaCl and 100µL samples were desalted using Zeba Spin Desalting Plates, 7K MWCO. Results were analyzed by BCA Protein Assay and conductivity measurements.
Protein Protein Recovery % NaCl Removed
BSA (66 kDa) 98.5 >95
α-Lactalbumin (14.1 kDa) 91.5 >95
Ubiquitin (8.6 kDa) 85 >95

The Zeba Desalting Cartridge-fittings are compatible with syringe processing or will attach to popular automated liquid-chromatography systems such as ÄKTA™ or FPLC Systems without additional connectors. Each Zeba Desalting Chromatography Cartridge includes an accessory pack that readily adapts cartridges for use with Luer-Lok™ Syringe Fittings or tubing. The cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses and efficiently process samples from 50 to 1500µL.

More Product Data

Related Resources:

Desalting and gel filtration (Technical Guide)
Desalting Products Selection Guide

Related Products:

Zeba Desalting Columns and Devices, 40K MWCO
Dialysis Tubing, Cassettes and Devices
Detergent Removal Resin, Columns and Plates
Pierce Protein Concentrators

Available Formats:

  • Spin Columns – 75µL, 0.5mL (microcentrifuge), 2mL, 5mL, 10mL columns
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing (learn more about our Chromatography Cartridges)
  • 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. (see Zeba 96-well Spin Plate data)

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89877 Zeba Micro Spin Desalting Columns, 7K MWCO, 75µL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 2 to 12µL
25 columns Product Instructions for product #89877 Zeba Micro Spin Desalting Columns, 7K MWCO, 75µL MSDS for product #89877 Zeba Micro Spin Desalting Columns, 7K MWCO, 75µL $126.00

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89878 Zeba Micro Spin Desalting Columns, 7K MWCO, 75µL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 50 samples, each 2 to 12µL
50 columns MSDS for product #89878 Zeba Micro Spin Desalting Columns, 7K MWCO, 75µL Certificate of Analysis for product #89878 Zeba Micro Spin Desalting Columns, 7K MWCO, 75µL $222.00

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89882 Zeba Spin Desalting Columns, 7K MWCO, 0.5mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 30 to 130µL
25 columns Product Instructions for product #89882 Zeba Spin Desalting Columns, 7K MWCO, 0.5mL MSDS for product #89882 Zeba Spin Desalting Columns, 7K MWCO, 0.5mL Certificate of Analysis for product #89882 Zeba Spin Desalting Columns, 7K MWCO, 0.5mL $115.00

Add to cart
89883 Zeba Spin Desalting Columns, 7K MWCO, 0.5mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 50 samples, each 30 to 130µL
50 columns MSDS for product #89883 Zeba Spin Desalting Columns, 7K MWCO, 0.5mL $207.00

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89883B Zeba Spin Desalting Columns, 7K MWCO, 0.5mL
Formulation: Polypropylene columns with proprietary resin
Custom MSDS for product #89883B Zeba Spin Desalting Columns, 7K MWCO, 0.5mL
89889 Zeba Spin Desalting Columns, 7K MWCO, 2mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 5 samples, each 200 to 700µL
5 columns Product Instructions for product #89889 Zeba Spin Desalting Columns, 7K MWCO, 2mL MSDS for product #89889 Zeba Spin Desalting Columns, 7K MWCO, 2mL Certificate of Analysis for product #89889 Zeba Spin Desalting Columns, 7K MWCO, 2mL $47.00

Add to cart
89890 Zeba Spin Desalting Columns, 7K MWCO, 2mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 200 to 700µL
25 columns Product Instructions for product #89890 Zeba Spin Desalting Columns, 7K MWCO, 2mL MSDS for product #89890 Zeba Spin Desalting Columns, 7K MWCO, 2mL $209.00

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89891 Zeba Spin Desalting Columns, 7K MWCO, 5mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 5 samples, each 500 to 2000µL
5 columns Product Instructions for product #89891 Zeba Spin Desalting Columns, 7K MWCO, 5mL MSDS for product #89891 Zeba Spin Desalting Columns, 7K MWCO, 5mL $60.00

Add to cart
89892 Zeba Spin Desalting Columns, 7K MWCO, 5mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 500 to 2000µL
25 columns MSDS for product #89892 Zeba Spin Desalting Columns, 7K MWCO, 5mL $267.00

Add to cart
89893 Zeba Spin Desalting Columns, 7K MWCO, 10mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 5 samples, each 700µL to 4mL
5 columns Product Instructions for product #89893 Zeba Spin Desalting Columns, 7K MWCO, 10mL MSDS for product #89893 Zeba Spin Desalting Columns, 7K MWCO, 10mL Certificate of Analysis for product #89893 Zeba Spin Desalting Columns, 7K MWCO, 10mL $74.00

Add to cart
89894 Zeba Spin Desalting Columns, 7K MWCO, 10mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 700µL to 4mL
25 columns MSDS for product #89894 Zeba Spin Desalting Columns, 7K MWCO, 10mL $323.00

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89807 Zeba Spin Desalting Plates, 7K MWCO
Formulation: Polypropylene high-profile 96-well filter plates containing 550µL resin; Polypropylene wash and collection plates
Sufficient For: 2 x 96 samples, each 20 to 100µL


Learn more about Pierce 96-well Filter Spin Plates.
2 plates Product Instructions for product #89807 Zeba Spin Desalting Plates, 7K MWCO MSDS for product #89807 Zeba Spin Desalting Plates, 7K MWCO Certificate of Analysis for product #89807 Zeba Spin Desalting Plates, 7K MWCO $236.00

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89808 Zeba Spin Desalting Plates, 7K MWCO
Formulation: Polypropylene high-profile 96-well filter plates containing 550µL resin; Polypropylene wash and collection plates
Sufficient For: 4 x 96 samples, each 20 to 100µL


Learn more about Pierce 96-well Filter Spin Plates.
4 plates Product Instructions for product #89808 Zeba Spin Desalting Plates, 7K MWCO MSDS for product #89808 Zeba Spin Desalting Plates, 7K MWCO Certificate of Analysis for product #89808 Zeba Spin Desalting Plates, 7K MWCO $428.00

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89934 Zeba Desalting Chromatography Cartridges, 7K MWCO, 1mL
Formulation: Polypropylene cartridge with proprietary resin
Sufficient For: samples requiring 1mL of resin for separation


Learn more about Pierce Chromatography Cartridges.
5 cartridges Product Instructions for product #89934 Zeba Desalting Chromatography Cartridges, 7K MWCO, 1mL MSDS for product #89934 Zeba Desalting Chromatography Cartridges, 7K MWCO, 1mL Certificate of Analysis for product #89934 Zeba Desalting Chromatography Cartridges, 7K MWCO, 1mL $165.00

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89935 Zeba Desalting Chromatography Cartridges, 7K MWCO, 5mL
Formulation: Polypropylene cartridge with proprietary resin
Sufficient For: samples requiring 5mL of resin for separation


Learn more about Pierce Chromatography Cartridges.
5 cartridges Product Instructions for product #89935 Zeba Desalting Chromatography Cartridges, 7K MWCO, 5mL MSDS for product #89935 Zeba Desalting Chromatography Cartridges, 7K MWCO, 5mL Certificate of Analysis for product #89935 Zeba Desalting Chromatography Cartridges, 7K MWCO, 5mL $190.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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