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Zeba Spin Desalting Columns and Devices, 40K MWCO


Quickly remove salts and small peptides from large proteins (high MW).

Zeba Spin Desalting Columns, Desalting Plates, Desalting Cartridges.

Thermo Scientific Zeba Spin Desalting Columns and Plates (40K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 5μL to 4mL samples.

Zeba Spin Desalting Columns and Plates are polypropylene devices containing a proprietary high-performance size-exclusion resin for effective removal of salts and other small molecules (< 2000 daltons) while providing excellent recovery for protein larger than 40,000 daltons. Available in 5 different spin columns sizes, 96-well filter spin plates, Zeba Desalting Devices provide trouble-free desalting and buffer exchange for sample volumes ranging from 5µL to 4mL. The easy-to-use spin column format dramatically improves results over standard drip-column methodologies and the 96-well spin plate format enables more high-throughput processing.

Highlights:

  • High recovery – low-binding resin maximizes protein recovery
  • Fast – no screening of fractions or waiting for protein to emerge by gravity-flow
  • Easy-to-use – no cumbersome column preparation or equilibration
  • Flexible – available in spin columns and filter spin plates for a range of needs
  • Economical – comparable performance at lower cost than other commercially available resins

Product Details:

Video of Zeba Spin Desalting Columns in action!

Zeba Spin Desalting Columns, 40K MWCO contain a proprietary high-performance desalting resin that provides exceptional desalting and protein-recovery characteristics compared to other commercially available resins. Dilute (25µg/mL) protein samples can be successfully processed to achieve greater than 90% retention (removal) of salts and other small molecules (< 2000 MW) while maintaining and good recovery of proteins and other macromolecules (> 40,000 MW). Zeba Spin Desalting Columns can be used to process samples from 5 to 4000µL.

Thermo Scientific Zeba 40K and 7K Desalt Resin Performance. Sample volumes of 100µL of different compounds were processed with 0.5mL of two Zeba Resins, as well as with two 30K-50K MWCO resins from Suppliers G and B. The Zeba Resins removed 85-100% of DTT, NaCl and biotin.
Tested Compound   Mass (Da) Concentration Loaded Percent Removal
Zeba 40K Resin Zeba 7K Resin Supplier G Resin Supplier B Resin
NaCl 58.44 1M 100 100 95 100
DTT 154 0.5M 100 99 76 97
Sulfo-NHS-LC-Biotin
557 0.27mM 88 87 72 77
Bacitracin 1200 0.5mg/mL 97 79 80 74
Vitamin B12 1386 0.5mg/mL 99 93 67 86

 


Zeba Desalting Spin Columns 40K and 7K MWCO compared to GE Healthcare and Bio-Rad Desalting Resins
 
The 40K resin effectively removes salts with excellent protein recovery. Comparison of the 40K MWCO Zeba Desalt Resin with two commercially available resins of similar MWCO (30K-50K). Two concentrations of BSA (66 kDa) were prepared in 1M NaCl. Samples (700µL or 200µL) were applied to 2mL desalting columns pre-equilibrated in water. The columns were centrifuged for 4 minutes at 1,000 x g. Desalted samples were tested for protein recovery (Thermo Scientific Pierce BCA Protein Assay, Part No. 23225) and conductivity. Samples were also resolved by SDS-PAGE and stained with Thermo Scientific Imperial Protein Stain (Part No. 24615) (A) or Pierce Silver Stain Kit (Part No. 24612) (B). The Zeba 40K Desalt Resin resulted in greater NaCl removal and higher protein recovery, especially at low protein concentrations and sample volumes.

The Zeba 96-well Spin Desalting Plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100µL) in 5 minutes.

Related Resources:

Desalting and gel filtration (Technical Guide)
Desalting Products Selection Guide

Related Products:

Zeba Spin Desalting Columns and Devices, 7K MWCO
Dialysis Tubing, Cassettes and Devices
Detergent Removal Resin, Columns and Plates
Pierce Protein Concentrators

Available Formats:

  • Spin Columns – 75µL, 0.5mL (microcentrifuge), 2mL, 5mL, 10mL columns
  • 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. (see Zeba 96-well Spin Plate data)

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
87764 Zeba Micro Spin Desalting Columns, 40K MWCO, 75µL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 5 to 14µL
25 columns Product Instructions for product #87764 Zeba Micro Spin Desalting Columns, 40K MWCO, 75µL MSDS for product #87764 Zeba Micro Spin Desalting Columns, 40K MWCO, 75µL $126.00

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87765 Zeba Micro Spin Desalting Columns, 40K MWCO, 75µL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 50 samples, each 5 to 14µL
50 columns MSDS for product #87765 Zeba Micro Spin Desalting Columns, 40K MWCO, 75µL $222.00

Add to cart
87766 Zeba Spin Desalting Columns, 40K MWCO, 0.5mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 70 to 200µL
25 columns Product Instructions for product #87766 Zeba Spin Desalting Columns, 40K MWCO, 0.5mL MSDS for product #87766 Zeba Spin Desalting Columns, 40K MWCO, 0.5mL $115.00

Add to cart
87767 Zeba Spin Desalting Columns, 40K MWCO, 0.5mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 50 samples, each 70 to 200µL
50 columns MSDS for product #87767 Zeba Spin Desalting Columns, 40K MWCO, 0.5mL $207.00

Add to cart
87768 Zeba Spin Desalting Columns, 40K MWCO, 2mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 5 samples, each 200 to 900µL
5 columns Product Instructions for product #87768 Zeba Spin Desalting Columns, 40K MWCO, 2mL MSDS for product #87768 Zeba Spin Desalting Columns, 40K MWCO, 2mL $47.00

Add to cart
87769 Zeba Spin Desalting Columns, 40K MWCO, 2mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 200 to 900µL
25 columns MSDS for product #87769 Zeba Spin Desalting Columns, 40K MWCO, 2mL $209.00

Add to cart
87770 Zeba Spin Desalting Columns, 40K MWCO, 5mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 5 samples, each 300 to 2000µL
5 columns Product Instructions for product #87770 Zeba Spin Desalting Columns, 40K MWCO, 5mL MSDS for product #87770 Zeba Spin Desalting Columns, 40K MWCO, 5mL $60.00

Add to cart
87771 Zeba Spin Desalting Columns, 40K MWCO, 5mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 300 to 2000µL
25 columns MSDS for product #87771 Zeba Spin Desalting Columns, 40K MWCO, 5mL $267.00

Add to cart
87772 Zeba Spin Desalting Columns, 40K MWCO, 10mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 5 samples, each 1mL to 4mL
5 columns Product Instructions for product #87772 Zeba Spin Desalting Columns, 40K MWCO, 10mL MSDS for product #87772 Zeba Spin Desalting Columns, 40K MWCO, 10mL $74.00

Add to cart
87773 Zeba Spin Desalting Columns, 40K MWCO, 10mL
Formulation: Polypropylene columns with proprietary resin
Sufficient For: 25 samples, each 1mL to 4mL
25 columns MSDS for product #87773 Zeba Spin Desalting Columns, 40K MWCO, 10mL $323.00

Add to cart
87774 Zeba 96-well Spin Desalting Plates, 40K MWCO
Formulation: Polypropylene high-profile 96-well filter plates containing resin; Polypropylene wash and collection plates Sufficient For: 2 x 96 samples, each 20 to 100µL

Learn more about Pierce 96-well Filter Spin Plates.
2 plates Product Instructions for product #87774 Zeba 96-well Spin Desalting Plates, 40K MWCO MSDS for product #87774 Zeba 96-well Spin Desalting Plates, 40K MWCO Certificate of Analysis for product #87774 Zeba 96-well Spin Desalting Plates, 40K MWCO $236.00

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87775 Zeba 96-well Spin Desalting Plates, 40K MWCO
Formulation: Polypropylene high-profile 96-well filter plates containing resin; Polypropylene wash and collection plates Sufficient For: 4 x 96 samples, each 20 to 100µL

Learn more about Pierce 96-well Filter Spin Plates.
4 plates Product Instructions for product #87775 Zeba 96-well Spin Desalting Plates, 40K MWCO MSDS for product #87775 Zeba 96-well Spin Desalting Plates, 40K MWCO $428.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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