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Whole Cell Fluorescent Stains


Four colors of fluorescent probes to stain whole cells for fluorescence microscopy.

Fluorescent whole cell stains for cellular imaging.

Thermo Scientific Whole Cell Stains are four colors of reactive fluorescent dyes that evenly bind to stain entire, fixed cells for cellular imaging analysis by fluorescence microscopy.

These blue, green, orange and red fluorescent stains for whole-cell labeling are intense, highly photostable and matched to the output wavelengths of common fluorescence instrumentation. Many high-content screening (HCS) methods and other cell-imaging assays based on fluorescence microscopy require labeling of entire cells alongside detection of specific targets. Whole cell stains are used to identify and count individual cells and to define the cell region in which image analysis is applied. The primary object might be a specific protein, the nucleus or other major cellular component, an organelle or whole cells.

Highlights:

  • Whole cell fluorescent stains – reactive dyes bind to cell surfaces and contents to provide complete and even visualization of fixed cells in fluorescence imaging applications
  • Options for multiplexing – four fluorescent color-options enable dual staining with nearly any fluorescent reporter or fluor-labeled antibody probe
  • Compatible – all four fluorescent dyes match emission and excitation filters and lasers used in standard fluorescence microscopy and imaging instruments

Product Details:

Four colors of fluorescent whole cell stains.
Four colors of fluorescent whole cell stains. NIH 3T3 cells stained with Thermo Scientific Whole Cell Stains (Blue, Green, Orange and Red, respectively) for 30 minutes. Cell images were acquired on the Thermo Scientific ArrayScan HCS Reader with a 20X objective lens.

Identify cell boundaries with whole cell stains.
Identify cell boundaries with whole cell stains. U2OS cells in sub-confluent culture conditions stained with Thermo Scientific Whole Cell Stain Green, allowing the cell boundary (red lines) to be identified on the image acquired on the Thermo Scientific ArrayScan HCS Reader with a 20X objective lens and analyzed using the Morphology Explorer BioApplication.

Related Resources:

Immunohistochemistry (IHC) technical guide
IHC counterstains technical guide

Related Products:

Protein Fluorescent Labeling Reagents
Immunohistochemistry Reagents – including DNA and nuclear fluorescent stains
Cellular Imaging and Analysis Reagents (formerly Cellomics Products)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
8403501 Whole Cell Stain Blue
Formulation: Lyophilized proprietary fluorescent dye, Ex.350nm, Em.450nm
Sufficient For: Approx. 1 x 96 microplate wells
1 × 96 vial Product Instructions for product #8403501 Whole Cell Stain Blue MSDS for product #8403501 Whole Cell Stain Blue Certificate of Analysis for product #8403501 Whole Cell Stain Blue $45.00

Add to cart
8403502 Whole Cell Stain Blue
Formulation: Lyophilized proprietary fluorescent dye, Ex.350nm, Em.450nm
Sufficient For: Approx. 5 x 96 microplate wells
5 × 96 vial Product Instructions for product #8403502 Whole Cell Stain Blue MSDS for product #8403502 Whole Cell Stain Blue Certificate of Analysis for product #8403502 Whole Cell Stain Blue $165.00

Add to cart
8403201 Whole Cell Stain Green
Formulation: Lyophilized proprietary fluorescent dye, Ex.493nm, Em.518nm
Sufficient For: Approx. 1 x 96 microplate wells
1 × 96 vial Product Instructions for product #8403201 Whole Cell Stain Green MSDS for product #8403201 Whole Cell Stain Green Certificate of Analysis for product #8403201 Whole Cell Stain Green $45.00

Add to cart
8403202 Whole Cell Stain Green
Formulation: Lyophilized proprietary fluorescent dye, Ex.493nm, Em.518nm
Sufficient For: Approx. 5 x 96 microplate wells
5 × 96 vial Product Instructions for product #8403202 Whole Cell Stain Green MSDS for product #8403202 Whole Cell Stain Green Certificate of Analysis for product #8403202 Whole Cell Stain Green $165.00

Add to cart
8403301 Whole Cell Stain Orange
Formulation: Lyophilized proprietary fluorescent dye, Ex.550nm, Em.568nm
Sufficient For: Approx. 1 x 96 microplate wells
1 × 96 vial Product Instructions for product #8403301 Whole Cell Stain Orange MSDS for product #8403301 Whole Cell Stain Orange Certificate of Analysis for product #8403301 Whole Cell Stain Orange $45.00

Add to cart
8403302 Whole Cell Stain Orange
Formulation: Lyophilized proprietary fluorescent dye, Ex.550nm, Em.568nm
Sufficient For: Approx. 5 x 96 microplate wells
5 × 96 vial Product Instructions for product #8403302 Whole Cell Stain Orange MSDS for product #8403302 Whole Cell Stain Orange Certificate of Analysis for product #8403302 Whole Cell Stain Orange $165.00

Add to cart
8403401 Whole Cell Stain Red
Formulation: Lyophilized proprietary fluorescent dye, Ex.654nm, Em.673nm
Sufficient For: Approx. 1 x 96 microplate wells
1 × 96 vial Product Instructions for product #8403401 Whole Cell Stain Red MSDS for product #8403401 Whole Cell Stain Red Certificate of Analysis for product #8403401 Whole Cell Stain Red $45.00

Add to cart
8403402 Whole Cell Stain Red
Formulation: Lyophilized proprietary fluorescent dye, Ex.654nm, Em.673nm
Sufficient For: Approx. 5 x 96 microplate wells
5 × 96 vial Product Instructions for product #8403402 Whole Cell Stain Red MSDS for product #8403402 Whole Cell Stain Red Certificate of Analysis for product #8403402 Whole Cell Stain Red $165.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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