Thermo Scientific Pierce Vacuum Hydrolysis Tubes are high-quality glass tubes designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.
Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition. The tubes are suitable vessels for conducting other chemistries that require vacuum conditions (e.g., hydrazinolysis, sample concentration or lyophilization techniques).
- Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
- Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
- Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
- Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules
- Protein/peptide hydrolysis
- Sample concentration
Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.
- Eveleigh, J.W., and Winter, G.D. (1970). Protein Sequence Determination, Ed. Needleman, S. B., Springer-Verlag, pp 92-5.
- Westall, F. and Hesser, W. (1974). Fifteen-minute acid hydrolysis of peptides. Anal Biochem 61:610-3.
- Penke, B., et al. (1974). New acid hydrolysis method for determining tryptophan in peptides and proteins. Anal Biochem 60:45-50.
- Creamer, L.K. and Matheson, A.R. N.Z.J. (1976). The use of mercaptoethanesulfonic acid as a hydrolyzing agent for the determination of tryptophan in proteins. Dairy Sci Technol 11:211-2.
- Liu, T.-Y. and Chang, Y.H. (1971). Hydrolysis of proteins with p-toluenesulfonic acid. J Biol Chem 246:2842-8.
- Simpson, R.J., et al. (1976). Complete amino acid analysis of proteins from a single hydrolysate. J Biol Chem 251:1936-40.
Reacti-Therm Heating Modules
Constant Boiling HCl (6N)
Trifluoroacetic Acid (TFA)