The Thermo Scientific TurboFect in vitro Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.
TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery into eukaryotic cells. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells. Transfection can be performed in the presence or absence of serum. TurboFect Reagent demonstrates superior transfection efficiency and minimal toxicity when compared to other lipid-based or polymer-based transfection reagents.
- High transfection efficiency of a wide variety of cell types
- No optimization required for most cell lines
- Excellent transfection efficiency in the presence or absence of serum
- Minimal cytotoxicity
- Ready-to-use – no need to reconstitute, dilute or manipulate
- Stable and transient transfection or co-transfection of DNA or RNA
- Experiments with adherent or suspension cells, differentiated or undifferentiated cells
- Gene expression studies and assays involving GFP or luciferase reporters
TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. As a result, rapid osmotic swelling of the endocytosed DNA-complex causes it to rupture in the cytosol, allowing translocation of DNA to the nucleus.
|Successful transfection of many cell types with Thermo Scientific TurboFect in vitro Transfection Reagent. Comparison of transfection efficiency in various cell types transfected with a plasmid DNA encoding eGFP using TurboFect Reagent. Transfections (n = 2 or 3) were analyzed by flow cytometry.
Cell types that have been successfully transfected with reporter genes using Thermo Scientific TurboFect Transfection Reagent.
|Permanently growing cell lines
||Primary cell cultures
Cos-7 — african green monkey kidney cells
HeLa — human cervix adenocarcinoma cells
CHO — chinese hamster ovary cells
HEK293 — human embryonic kidney cells
B50 — rat nervous tissue neuronal cells
Calu1 — human lung epidermoid carcinoma cells
RAW264 — mouse leukaemic monocyte-macrophage cells
WEHI — mouse B cell lymphoma cells
MDCK — Madin Darby Canine Kidney cells
Raji — human Burkitt's lymphoma cells
COLO — human colon adenocarcinoma cells
Jurkat — human leukaemic T cells
Sp2/Ag14 — mouse myeloma cells
HeLa S3 — human cervix carcinoma cells
Hep2C — human larynx carcinoma cells
L929 — mouse connective tissue fibroblasts
NIH3T3 — mouse embryo fibroblasts
- Rat fibroblasts
- Mouse bone marrow derived dendritic cells
- Mouse bone marrow derived macrophages
- HLF human lung fibroblasts
|Better transfection with Thermo Scientific TurboFect in vitro Transfection Reagent. Comparison of transfection efficiency (left axis) and protein expression (right axis) in HepG2 cells transfected with a plasmid DNA encoding eGFP using TurboFect Reagent or competing products from other suppliers. Transfections were performed (n = 2 or 3) according to each manufacturer’s recommendations and analyzed by flow cytometry.
Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide
Pierce Luciferase Assay Kits and Vectors
Human in vitro Transcription Kits and Reagents