Update profile
    Change password
    Sign out

Our content has moved! Check out our protein biology page on lifetechnologies.com  Go now >

TSAT (Tris-succinimidyl aminotriacetate)

For three-way crosslinking between primary amine molecules.

Pierce TSAT

Thermo Scientific Pierce TSAT is tris-(succinimidyl) aminotriacetate, a novel trifunctional crosslinker that contains three short spacers arms linked to a central nitrogen atom, each ending in an amine-reactive NHS-ester group.


  • Reactive groups: NHS ester (three ends)
  • Reactive towards: amino groups (primary amines)
  • NHS ester couples to amines at pH 7-9 form stable amide bonds
  • Trifunctional amine-reactive crosslinking agent
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO)


  • Preparation of multicomponent aggregates
  • Polyerization of protein interactions for nearest-neighbor studies
  • Core molecule for the construction of dendritic polymers

Product Details:

Tris-succinimidyl aminotriacetate; TSAT
Chemical structure of TSAT crosslinking reagent.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
TMEA (trifunctional sulfhydryl-reactive crosslinker)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
33063 TSAT
Formulation: Tris-succinimidyl aminotriacetate; solid
Sufficient For: 104mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #33063 TSAT MSDS for product #33063 TSAT Certificate of Analysis for product #33063 TSAT $142.00

Now available on lifetechnologies.com

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
89870 - Pierce C18 Spin Columns

22322 - Sulfo-SMCC

89900 - RIPA Lysis and Extraction Buffer

23235 - Micro BCA Protein Assay Kit

23228 - BCA Protein Assay Reagent A


Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2015 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747