Thermo Scientific Pierce 0.1% Trifluoroacetic Acid (v/v) in Water is an LC/MS-grade preparation with high purity and low UV-absorptivity that is ideal for HPLC and mass spectrometry applications.
This 0.1% Trifluoroacetic Acid in Water is specially purified by a proprietary method and tested to ensure lot-to-lot consistency with a low UV absorbance, providing the most sensitive detection across wavelengths and prolonging equipment life. The chromatography- and spectrometry-grade 0.1% TFA in water is 0.2-micron filtered, packaged in solvent-rinsed amber glass bottles and sealed under a nitrogen with PTFE-lined fluorocarbon caps for ultimate protection.
- Validated – tested more than 20 ways to ensure maximal LC/MS sensitivity
- Purified – processed, filtered and sealed to extend LC/MS column life
- Convenient – packaged to eliminate variability and reduce handling
General properties of trifluoroacetic acid (TFA).
|Melting / boiling point
||-15°C / 72°C
This preparation of Thermo Scientific 0.1% Trifluoroacetic Acid (v/v) in Water is specially purified and tested to the highest specifications to ensure the integrity of chromatography data, maximize assay sensitivity and prolong the life of equipment.
Specifications of Thermo Scientific 0.1% Trifluoroacetic Acid (v/v) in Water.
- Protease: Not Detected
- Trifluoroacetic Acid content: 0.095 to 0.105%
- Residue after Evaporation: <=1ppm
- Color: <=10 ALPHA
- LC-MS Gradient Suitability: Passes
- Identification: Passes
- UV Absorbance (au):
- 210nm <=0.55
- 220nm <=0.18
- 230nm <=0.06
- 254nm <=0.003
- Trace Ionic Impurities:
- Aluminum (Al) <=20ppb
- Calcium (Ca) <=50ppb
- Copper (Cu) <=10ppb
- Iron (Fe) <=10ppb
- Lead (Pb) <=10ppb
- Magnesium (Mg) <=10ppb
- Manganese (Mn) <=10ppb
- Nickel (Ni) <=10ppb
- Potassium (K) <=20ppb
- Silver (Ag) <=10ppb
- Sodium (Na) <=50ppb
- Zinc (Zn) <=20ppb
For complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptides that would otherwise overlap. Trifluoroacetic acid is the most frequently used modifier for peptide separations in reverse-phase HPLC. The TFA concentration usually specified is 0.1%. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make TFA concentrations the same.
All TFA and Formic Acid formulations (Acetonitrile, Water)
Heptafluorobutyric acid (HFBA)
Water (LC-MS Grade)