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Trifluoroacetic Acid (0.1%) in Acetonitrile


Convenient, pre-diluted solvent blend for mass spectrometry applications.

Pierce 0.1% Trifluoroacetic Acid in Acetonitrile

Thermo Scientific Pierce 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile is an LC/MS-grade preparation with high purity and low UV-absorptivity that is ideal for HPLC and mass spectrometry applications.

This 0.1% Trifluoroacetic Acid in Acetonitrile is specially purified by a proprietary method and tested to ensure lot-to-lot consistency with a low UV absorbance, providing the most sensitive detection across wavelengths and prolonging equipment life. The chromatography- and spectrometry-grade 0.1% TFA in acetonitrile is 0.2-micron filtered, packaged in solvent-rinsed amber glass bottles and sealed under a nitrogen with PTFE-lined fluorocarbon caps for ultimate protection.

Highlights:

  • Validated – tested more than 20 ways to ensure maximal LC/MS sensitivity
  • Purified – processed, filtered and sealed to extend LC/MS column life
  • Convenient – packaged to eliminate variability and reduce handling

Product Details:

Chemical structure of Trifluoroacetic acid

General properties of trifluoroacetic acid (TFA).
Alternative names Perfluoroacetic acid,
Trifluoroethanoic acid,
Trifluoracetic acid
Molecular formula CF3COOH
Molecular weight 114.02
Density 1.53g/mL, 20°C
Melting / boiling point -15°C / 72°C
CAS number 76-05-01

This preparation of Thermo Scientific 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile is specially purified and tested to the highest specifications to ensure the integrity of chromatography data, maximize assay sensitivity and prolong the life of equipment.

Specifications of Thermo Scientific 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile.
  • Trifluoroacetic Acid content: 0.095 to 0.105%
  • Water Content <=0.01%
  • Residue after Evaporation: <=1ppm
  • Color: <=10 ALPHA
  • LC-MS Gradient Suitability: Passes
  • Identification: Passes
  • UV Absorbance (au):
    • 210nm <=0.6
    • 220nm <=0.55
    • 230nm <=0.4
    • 254nm <=0.03
  • Trace Ionic Impurities:
    • Aluminum (Al) <=25ppb
    • Calcium (Ca) <=50ppb
    • Copper (Cu) <=10ppb
    • Iron (Fe) <=10ppb
    • Lead (Pb) <=10ppb
    • Magnesium (Mg) <=10ppb
    • Manganese (Mn) <=10ppb
    • Nickel (Ni) <=10ppb
    • Potassium (K) <=20ppb
    • Silver (Ag) <=10ppb
    • Sodium (Na) <=50ppb
    • Zinc (Zn) <=20ppb

For complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptides that would otherwise overlap. Trifluoroacetic acid is the most frequently used modifier for peptide separations in reverse-phase HPLC. The TFA concentration usually specified is 0.1%. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make TFA concentrations the same.

Related Products:

All TFA and Formic Acid formulations (Acetonitrile, Water)
Dimethylsulfoxide (DMSO)
Heptafluorobutyric acid (HFBA)
Triethylamine
Water (LC-MS Grade)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
85176 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade
Formulation: Clear, colorless solution of TFA (low metal; low water; low absorbance; 0.2μm-filtered in amber glass bottles)
Sufficient For: Liquid chromatography and mass spectrometry applications
1L MSDS for product #85176 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade Certificate of Analysis for product #85176 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade $135.00

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85177 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade
Formulation: Clear, colorless solution of TFA (low metal; low water; low absorbance; 0.2┬Ám-filtered in amber glass bottles)
Sufficient For: Liquid chromatography and mass spectrometry applications
4 x 1L MSDS for product #85177 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade Certificate of Analysis for product #85177 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade $477.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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