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Total GSK-3 beta ELISA Kit


Microplate sandwich ELISA kit for total GSK-3 beta (pGSK-3b and GSK-3b).

Thermo Scientific Total GSK-3 beta ELISA Kits

Assay total GSK-3β (pan glycogen synthase kinase 3 beta, GSK-3b + pGSK-3b) levels in cell lysates of human and rat origin with our Thermo Scientific Total GSK-3 beta ELISA Kit.

This GSK-3 beta ELISA kit is an immunoassay for quantitative determination of glycogen synthase kinase 3 beta (pan or total GSK-3β) in cell lysates. A recombinant GSK-3β standard is provided in the kit. The kit uses a monoclonal antibody to GSK-3β precoated in a microplate to bind the GSK-3β in the standards or sample. After a short incubation, the excess sample or standard is washed out and a rabbit polyclonal antibody to GSK-3β is added. This antibody binds to GSK-3β captured on the plate. The polyclonal antibody detects a non-phosphorylated region of GSK-3β and therefore detects GSK-3β regardless of phosphorylation status. After a short incubation, the excess antibody is washed out and secondary conjugate is added, which binds to the polyclonal GSK-3β antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450nm. The measured optical density is directly proportional to the concentration of GSK-3β in either standards or samples.

Highlights:

  • Target: phosphorylated GSK-3β ([pSer9] glycogen synthase kinase 3 beta, pGSK-3β)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 78.1 to 5000pg/mL
  • Sensitivity: < 74.4pg/mL
  • Precision: dependent upon sample type and level (see instructions)
  • Sample Types: cell lysates of human, mouse, and rat origin
  • Total Assay Time: 3 hours
  • Sample Size: 100μL
  • Specificity: no significant cross-reaction with GSK-3α, ATP Citrate Lyase, MEK-1, AKT, phospho-JNK, ERK and phospho-ERK.
Video of the Thermo Scientific Total GSK-3 beta ELISA Kit

Includes:

Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, standard, substrate, and stop solution.

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Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMSGSK3T Total GSK-3 beta ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Precoated 96-well Plate
Conjugate
Antibody
Assay Buffer 21
Wash Buffer Concentrate
Standard
TMB Substrate
RIPA Cell Lysis Buffer 2
Stop Solution 2
1-plate kit MSDS for product #EMSGSK3T Total GSK-3 beta ELISA Kit $499.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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