Thermo Scientific Pierce Thiophilic Adsorbent is a protein-free immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human.
Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups. The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate. In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples. Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH. Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions. The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity. The resin is available as both a standalone product and as a purification kit containing pre-packed columns and ready-to-use buffers.
- High capacity – gently bind and purify approx. 20mg polyclonal antibody per milliliter of adsorbent resin
- Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY
- Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps
- Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody
Thiophilic adsorption is based on the ability of some proteins, particularly immunoglobulins, to bind to an immobilized ligand that contains a sulfone group in close proximity to a thioether. Salts that interact with water molecules, such as potassium and ammonium sulfate, promote this binding by driving the protein into close proximity with the ligand.
Antibody yields and purity levels using Thermo Scientific Pierce Thiophilic Adsorbent.
||Total A280 Bound
||% Purity by HPLC
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- Nopper, B., Kohen, F. and Wilchek, M. (1989). A thiophilic adsorbent for the one-step high-performance liquid chromatography purification of monoclonal antibodies. Anal. Biochem. 180, 66-71.
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Review of antibody purification methods
Melon Gel Monoclonal IgG Purification Kit (for culture supernatant and ascites)
Melon Gel IgG Purification Kit (for serum samples)
Protein A, Protein G and other IgG purification resins