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TFCS Reagent

Temporarily blocks amine groups and extends lysine side chains.

Pierce TFCS

Thermo Scientific Pierce TFCS forms amide bonds with primary amines (e.g., lysine side chains) to add a seven-atom spacer arm (7.7 angstroms) terminated by a trifluoroacetyl group that can be removed later to expose a new primary amine.

TFCS ( N-[ε-trifluoroacetylcaproyloxy]succinimide ester) is an amine-reactive modification reagent with a protected primary amine group. This reagent is used to temporarily block amine groups in target molecules and to extend lysine side chains to reduce steric hindrance. The coupling reaction is performed in phosphate-buffered saline at pH 7.2, which also preserves the amine protecting group (i.e., trifluoroacetyl). The trifluoroacetyl is removed at pH 7.8-8.1 in phosphate or borate buffer.


  • Add a protected primary amine group
  • Extend lysine side-chain length to reduce steric hindrance
  • Temporarily block amine groups
  • Unmask the amino group to react with any amine-reactive crosslinker

Product Details:

TFCS Reagent N-(e-Trifluoroacetylcaproyloxy)succinimide ester

Structure and properties of TFCS:

  • Functional groups: NHS ester (amine-reactive), primary amine (protected)
  • Chemical name: N-(ε-Trifluoroacetylcaproyloxy)succinimide ester
  • Chemical formula: C12H15N2O5
  • Molecular weight: 324.25
  • Spacer arm: 7.7 angstroms
  • Form: solid, powder

Related Resources:

Overview of crosslinking and protein modification

Related Products:

Sulfo-NHS-Acetate – permanently block primary amines
Citraconic Anhydride – reversibly block primary amines
MS(PEG)n – block and pegylated primary amines

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22299 TFCS
Formulation: N-epsilon-Trifluoroacetylcaproyl-oxysuccinimide ester; solid
Sufficient For: 154mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22299 TFCS MSDS for product #22299 TFCS Certificate of Analysis for product #22299 TFCS $113.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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