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Premium Grade TCEP-HCl


Thoroughly tested, highly characterized and fully documented batches of reagent.

Pierce Premium Grade TCEP-HCl

Thermo Scientific Pierce Premium Grade TCEP-HCl is our highest quality formulation of this disulfide reducing agent, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade SPDP provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. TCEP-HCl is pure, crystalline Tris(2-carboxyethyl)phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable. TCEP selectively and completely reduces even the most stable water-soluble alkyl disulfides over a wide pH range.

Highlights:

  • TCEP – a thiol-free, odorless yet potent reducing agent for cleaving disulfide bonds
  • High quality – identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity – enhanced level of testing and characterization compared to standard grade
  • Lot retention – ample supply of past lots retained to ensure future process testing
  • Change management – Change Control Notification (CCN) service
  • Consistent manufacture – batch-specific manufacturing documentation review
Structure of TCEP-HCl (Tris[2-carboxyethyl]phosphine hydrochloride)

Advantages of TCEP-HCl over other reducing agents:

  • Odorless – Unlike DTT or BME, TCEP is odor-free, so reductions can be carried out conveniently on the bench top.
  • Stable in air – The inherent stability of the TCEP moiety eliminates the need for any special precautions to avoid oxidation when handling, using or storing TCEP.
  • Efficient – For most applications, 5 to 50mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within a few minutes at room temperature.
  • Compatible – With TCEP, removal of the reducing agent is not necessary prior to most applications, (e.g. histidine-tagged protein purification, maleimide conjugations).

Product Details:

Specifications of Premium Grade TCEP·HCl. We manufacture TCEP·HCl to the highest possible level of quality to ensure the integrity of your data and to provide you with the highest degree of consistency.
Test Premium Grade Specification Standard Grade
Purity Greater than 95% by quantitative NMR using an internal standard (the highest standard for crosslinker purity) Yes
Visual Snow white crystalline solid, free of foreign material Yes
Solubility Dissolves at 310 g/L in DI water to give a clear, colorless solution Yes
Identity IR scan shows only peaks characteristic of the structure and functional groups of TCEP-HCl
Functional assay TCEP-HCl reduces ≥ 90% of DNTB
Water content Karl Fischer titration, ≤ 2%
Trace metals All metals, including lead, ≤ 50 ppm
Elemental analysis Reported values for C, H, N, O, and S

Related Resources:

Overview of crosslinking and protein modification
Overview of protein electrophoresis

Related Products:

Premium Grade Reagents – complete menu
TCEP-HCl (standard grade)
Bond-Breaker TCEP Solution
Immobilized TCEP Disulfide Reducing Gel
All Disulfide Reducing Agents
Ellman’s Reagent – measure sulfhydryls


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
PG82080 Premium Grade TCEP-HCl
Formulation: Crystalline powder of Tris(2-carboxyethyl)phosphine hydrochloride
Sufficient For: Preparing nearly 70mL of 50mM solution
1g Product Instructions for product #PG82080 Premium Grade TCEP-HCl MSDS for product #PG82080 Premium Grade TCEP-HCl Certificate of Analysis for product #PG82080 Premium Grade TCEP-HCl $70.00

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PG82089 Premium Grade TCEP-HCl
Formulation: Crystalline powder of Tris(2-carboxyethyl)phosphine hydrochloride
Sufficient For: Preparing nearly 0.7L of 50mM solution
10g Product Instructions for product #PG82089 Premium Grade TCEP-HCl MSDS for product #PG82089 Premium Grade TCEP-HCl Certificate of Analysis for product #PG82089 Premium Grade TCEP-HCl $445.00

Add to cart
PG82090 Premium Grade TCEP-HCl
Formulation: Crystalline powder of Tris(2-carboxyethyl)phosphine hydrochloride
Sufficient For: Preparing nearly 7L of 50mM solution
100g Product Instructions for product #PG82090 Premium Grade TCEP-HCl MSDS for product #PG82090 Premium Grade TCEP-HCl Certificate of Analysis for product #PG82090 Premium Grade TCEP-HCl $2,880.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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