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Tandem Mass Tag Antibody and Enrichment Resin


Detect or purify peptides or proteins that have been labeled with TMT Reagents.

Antibody and affinity resin for Tandem Mass Tag Reagents

The Thermo Scientific Pierce Anti-TMT Antibody Resin and standalone antibody enable selective enrichment of peptides in complex samples that have been labeled with Tandem Mass Tag™ Reagents.

Tandem Mass Tag™ (TMT™) Reagents are used to multiplex-label and measure proteins in different samples using tandem mass spectrometry. The Anti-TMT Resin is agarose-immobilized anti-TMT monoclonal antibody, which is effective to capture and affinity purify peptides or proteins that have been labeled using any TMT Reagent. The strategy is effective for simplifying otherwise complex samples before running the mass analysis, especially when the targets for labeling are rare (e.g., cysteine sulfhydryls). Enrichment of labeled peptides by this method results in fewer peptides identified per protein but can significantly increase the number of unique proteins identified, making it easier to detect and measure low-abundance proteins.

Highlights:

  • Specific – uses specific, high-affinity antibody for the TMT structure (Kd >100pM)
  • Compatible – effective for all varieties of TMT Reagents (amine-reactive or cysteine-reactive)
  • Versatile – use complete kit for sample enrichment to simplify analysis, or choose the standalone resin or standalone antibody to develop your own purification and detection strategies

Product Details:

More Product Data

Related Products:

Tandem Mass Tag Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
90075 Anti-TMT Antibody
Formulation: Mouse IgG2b monoclonal antibody in PBS with BSA and preservative
Sufficient For: Detection of the TMT label in Western blots
0.1mL MSDS for product #90075 Anti-TMT Antibody Certificate of Analysis for product #90075 Anti-TMT Antibody $335.00

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90076 Immobilized Anti-TMT Antibody Resin
Formulation: Antibody on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding 250μg TMT-BSA/mL resin
6mL Product Instructions for product #90076 Immobilized Anti-TMT Antibody Resin MSDS for product #90076 Immobilized Anti-TMT Antibody Resin Certificate of Analysis for product #90076 Immobilized Anti-TMT Antibody Resin $450.00

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90104 TMT Elution Buffer
Formulation: Proprietary aqueous buffer, pH 7.3
Sufficient For: Elution of TMT-labeled peptides from immobilized anti-TMT antibody resin
20mL Product Instructions for product #90104 TMT Elution Buffer MSDS for product #90104 TMT Elution Buffer Certificate of Analysis for product #90104 TMT Elution Buffer $62.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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