Ten different high purity detergents for procedure testing and optimization.
The Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.
The sampler kit is an economical way to test which detergent is most effective for a particular protein storage or experimental system. Thermo Scientific Surfact-Amps Purified Detergent Solutions are convenient 10% (w/v) solutions of commonly used detergents that provide unsurpassed purity, quality and stability for proteomics and molecular biology methods. Unlike neat detergent formulations, Surfact-Amps 10% Solutions are not so viscous that you can't aliquot them accurately. The detergent solutions are carefully prepared and packaged under nitrogen in glass ampules, ensuring their stability and eliminating the accumulation of peroxides and degradation products. Three of the ten detergents in the sampler kit are supplied as solids (100mg each) rather than 10% solutions.
Surfact-Amps Detergent Solutions are:
Precise 10% detergent solutions in ultrapure water ampuled under nitrogen
Easy to accurately dispense and dilute for use
Exceptionally pure: less than 1.0µeq/mL peroxides and carbonyls
Highly stable: packaged in glass ampules under inert nitrogen gas
Detergents are a class of molecules whose unique properties enable manipulation (disruption or formation) of hydrophobic-hydrophilic interactions among molecules in biological samples. In biological research, detergents are used to lyse cells (release soluble proteins), solubilize membrane proteins and lipids, control protein crystallization, prevent nonspecific binding in affinity purification and immunoassay procedures, and as additives in electrophoresis. Generally, moderate concentrations of mild (i.e., nonionic) detergents compromise the integrity of cell membranes, thereby facilitating lysis of cells and extraction of soluble protein, often in native form. Using other conditions, detergents effectively penetrate between the membrane bilayers at concentrations sufficient to form mixed micelles with isolated phospholipids and membrane proteins.