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SuperSignal West Pico Chemiluminescent Substrate


Twice the signal duration and sensitivity of entry-level ECL substrates.

SuperSignal West Pico Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for low-picogram-level detection by Western blot analysis.

SuperSignal West Pico Substrate emits light for longer and chemiluminescent signal at more than twice the intensity of other entry-level luminol-based horseradish peroxidase detection systems while also maintaining sensitivity and low background. In addition, the greater signal intensity is achieved using less primary and secondary antibody than typical ECL substrates. All of this for about 30% less cost than comparable products.

Highlights:

  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes
  • Economy – costs less per milliliter than other similarly sensitive chemiluminescent substrates
  • Picogram sensitivity – highly sensitive for the rapid development of a wide range of protein levels
  • Stable light output – long-duration signal makes it possible to make multiple exposures to film
  • High intensity – signal is twice as strong as output from other luminol-based systems
  • Excellent stability – 24-hour working solution stability; 1-year kit stability at room temperature
  • Saves antibody – requires much less (more dilute) primary and secondary antibodies per blot

Product Details:

SuperSignal West Pico Chemiluminescent Substrate is stable for at least one year at room temperature and can be stored at 4°C for longer stability. The working solution is stable for a minimum of 24 hours at room temperature and can be used in either light or dark conditions.

In side-by-side comparisons using identical conditions, blots incubated in SuperSignal West Pico Chemiluminescent Substrate exhibit at least twice the intensity of blots treated with the GE Healthcare ECL System. SuperSignal West Pico Chemiluminescent Substrate costs much less than the GE Healthcare ECL System. Furthermore, the SuperSignal West Pico Substrate stock solution is room temperature (RT) stable for more than 12 months, with no discernable loss in activity. RT stability frees up valuable cold room space and saves time because there is no need to wait for the reagents to warm up. With signal duration of more than six hours, there is adequate time to optimize the exposure conditions. In most cases, there is no need to rerun gels. Switching to SuperSignal West Pico Substrate from an entry-level ECL substrate is not only easy, it will give you more of the results you are looking for.

Intensity of SuperSignal West Pico Substrate versus GE Healthcare ECL System 6 hours after incubation Enhance light emission kinetics: Thermo Scientific SuperSignal Substrate vs. GE ECL System. Net relative intensity six hours after incubation is much greater for SuperSignal West Pico Substrate than for the GE ECL System.

 

Western blots of IL-2 comparing detection limits of SuperSignal West Pico and Amersham ECL
Thermo Scientific SuperSignal West Pico is more sensitive than GE ECL Substrate. Recombinant mouse IL-2 was diluted (50 to 0.003ng) and electrophoresis was performed. The gels were transferred to nitrocellulose membranes, blocked and incubated with a 1µg/mL dilution of rat anti-mouse IL-2. After washing, the membranes were incubated with 20ng/mL dilutions of HRP-conjugated goat anti-rat antibody. The membranes were washed again and then incubated with substrate that was prepared according to the manufacturers' instructions. Blots were exposed to film for one- and five-minute exposures.

 

SuperSignal West Pico Substrate vs. Bio-Rad Clarity Substrate
Different substrates, different sensitivities. Direct performance comparison of three Thermo Scientific ECL substrates (panels A, B and D) to a competing substrate product from Bio-Rad™ Laboratories, Inc. (panel C). Blots are of identical dilution-series of HeLa cell extracts, probed for Hsp70, detected with optimized antibody dilutions and incubation conditions for each substrate, and exposed to X-ray film. Contrary to certain claims, no single substrate provides sensitivity and affordability for all ranges of detection. SuperSignal West Pico Substrate and Clarity™ Western ECL Substrate have similar performance and price points. By contrast, Pierce ECL Plus and other SuperSignal Substrates (Dura and Femto), provide greater sensitivity and/or signal duration than either SuperSignal West Pico Substrate or Clarity Substrate.

Related Resources:

Western blotting and detection technical guide
Western blot video tutorials

Related Products:

SuperSignal (and other) Western Blotting Kits – include secondary antibodies, etc.
Pierce Fast Western Blot Kits with SuperSignal Substrates
Selection Guide for Chemiluminescent Western Blot Substrates
Blotting Equipment, Membranes and Signal Enhancers
CL-XPosure Film (X-ray film for chemiluminescence)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
34079 SuperSignal West Pico Trial Kit
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 6 mini-gel blots; 500cm2 of membrane

Kit Contents:
Luminol/Enhancer, 25mL
Stable Peroxide Buffer, 25mL
50mL kit Product Instructions for product #34079 SuperSignal West Pico Trial Kit MSDS for product #34079 SuperSignal West Pico Trial Kit Certificate of Analysis for product #34079 SuperSignal West Pico Trial Kit $57.00

Add to cart
34077 SuperSignal West Pico Chemiluminescent Substrate
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 12 mini-gel blots; 1000cm2 of membrane

Kit Contents:
Luminol/Enhancer, 50mL
Stable Peroxide Buffer, 50mL
100mL kit Product Instructions for product #34077 SuperSignal West Pico Chemiluminescent Substrate MSDS for product #34077 SuperSignal West Pico Chemiluminescent Substrate Certificate of Analysis for product #34077 SuperSignal West Pico Chemiluminescent Substrate $85.00

Add to cart
34087 SuperSignal West Pico Chemiluminescent Substrate
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 25 mini-gel blots; 2000cm2 of membrane

Kit Contents:
Luminol/Enhancer, 100mL
Stable Peroxide Buffer, 100mL
200mL kit Product Instructions for product #34087 SuperSignal West Pico Chemiluminescent Substrate MSDS for product #34087 SuperSignal West Pico Chemiluminescent Substrate Certificate of Analysis for product #34087 SuperSignal West Pico Chemiluminescent Substrate $146.00

Add to cart
34080 SuperSignal West Pico Chemiluminescent Substrate
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 60 mini-gel blots; 5000cm2 of membrane

Kit Contents:
Luminol/Enhancer, 250mL
Stable Peroxide Buffer, 250mL
500mL kit Product Instructions for product #34080 SuperSignal West Pico Chemiluminescent Substrate MSDS for product #34080 SuperSignal West Pico Chemiluminescent Substrate Certificate of Analysis for product #34080 SuperSignal West Pico Chemiluminescent Substrate $222.00

Add to cart
34080PROMO SuperSignal West Pico Chemiluminescent Substrate
Formulation: 4 x Part No. 34080

Promotional item; expires June 30, 2014
4 x 500mL kit $666.00

Add to cart
34078 SuperSignal West Pico Chemiluminescent Substrate
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 120 mini-gel blots; 10,000cm2 of membrane

Kit Contents:
Luminol/Enhancer, 500mL
Stable Peroxide Buffer, 500mL
1L kit Product Instructions for product #34078 SuperSignal West Pico Chemiluminescent Substrate MSDS for product #34078 SuperSignal West Pico Chemiluminescent Substrate Certificate of Analysis for product #34078 SuperSignal West Pico Chemiluminescent Substrate $380.00

Add to cart
34078PROMO SuperSignal West Pico Chemiluminescent Substrate
Formulation: 4 x Part No. 34078

Promotional item; expires June 30, 2014
4 x 1L kit $1,140.00

Add to cart
34080AB SuperSignal West Pico Chemiluminescent Substrate
Formulation: Proprietary luminol and peroxide solutions

Kit Contents:
Luminol/Enhancer
Stable Peroxide Buffer
Custom MSDS for product #34080AB SuperSignal West Pico Chemiluminescent Substrate

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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