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SuperSignal West Dura Chemiluminescent Substrate


Long signal duration and high signal intensity, ideal for CCD cameras.

SuperSignal West Dura Chemiluminescent Western Blot Substrate for long-duration, stable light emission.

Thermo Scientific™ SuperSignal™ West Dura Substrate is a luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled-CCD camera detection systems. Unlike other substrates, whose signal declines to undetectable levels in 30 to 60 minutes, the signal produced with SuperSignal West Dura Substrate is stable for several hours, allowing multiple camera (or film) exposures to be made.

Highlights:

  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes
  • 24-hour signal – light emission is stable for 10 times longer than with typical ECL substrates; make multiple exposures to obtain publication-quality blot images
  • Great sensitivity – provides femtogram-level detection; see bands you've never been able to see before
  • Saves on antibody – works best with very dilute antibody concentrations, allowing you to perform 25 to 50 times more blots than with other chemiluminescent substrates
  • High intensity – immediate stable signal generation provides easy detection with compatible imaging system (or x-ray autoradiography film)
  • Reagent stability – 24-hour working solution stability; 1-year kit stability at room temperature

Product Details:

SuperSignal West Dura Substrate compared to Amersham ECL Prime
Thermo Scientific SuperSignal West Dura provides similar sensitivity and more stable signal duration compared to Amersham™ ECL Prime Substrate. Detection of Hsp86 in HeLa cell lysate on nitrocellulose membranes. HeLa cell lysate was diluted in sample buffer and heated to 95˚C for 5 minutes. Lane 1 contained 10μg total protein (total 10μL/well). Four 1:1 serial dilutions were then prepared and loaded at 10μL/well (Lanes 2-5). After transfer to nitrocellulose membranes (Part No. 88024), blots were blocked (Part No. 37542), probed with rabbit anti-Hsp 86 antibody (Part No. PA3-013) at 1:2000 dilution, followed by goat anti-rabbit IgG HRP secondary antibody (Part No. 31460) at 6.6ng/mL. Finally, respective blots were incubated with SuperSignal West Dura Substrate (Part No. 34076) or ECL Prime (GE Healthcare, Part No. RPN2232) per product instructions. At various time-points following substrate incubation, the two blots were imaged using a CCD camera imager. (Identical imaging exposure parameters were used for both blots at each time-point.)

SuperSignal West Dura Substrate compared to Bio-Rad Clarity Western ECL Substrate
SuperSignal West Dura is more sensitive than Bio-Rad™ Clarity™ Western ECL Substrate. Detection of MAP Kinase (Erk 1/2) and Hsp86 proteins in HeLa cell lysate on PVDF membranes. Membranes were probed, processed and exposed to X-ray film using identical conditions, except that incubations in substrates were according to respective manufacturer's product instructions.

Along with the advances in cooled CCD technology, lower cost of imaging systems and the desire to eliminate chemical waste associated with film development, it is increasingly common to see imaging instruments in research labs. Cooled CCD cameras allow instant image manipulation, higher sensitivity and a larger dynamic range than film. Use of imagers eliminates the need for film processing equipment and darkrooms. SuperSignal West Dura Extended Duration Substrate was developed for use with cooled CCD imaging systems, such as the Thermo Scientific myECL Imager (Part No. 62236).

More Product Data

Related Resources:

myECL Imager
Western blotting and detection technical guide
Western blot video tutorials

Related Products:

Pierce Fast Western Blot Kits with SuperSignal Substrates
Selection Guide for Chemiluminescent Western Blot Substrates
Blotting Equipment, Membranes and Signal Enhancers
CL-XPosure Film (X-ray film for chemiluminescence)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
37071 SuperSignal West Dura Substrate Trial Kit
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 2 mini-gel blots; 200cm2 of membrane

Kit Contents:
Luminol/Enhancer, 10mL
Stable Peroxide Buffer, 10mL
20mL kit Product Instructions for product #37071 SuperSignal West Dura Substrate Trial Kit MSDS for product #37071 SuperSignal West Dura Substrate Trial Kit Certificate of Analysis for product #37071 SuperSignal West Dura Substrate Trial Kit $100.00

Add to cart
34075 SuperSignal West Dura Extended Duration Substrate
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 12 mini-gel blots; 1000cm2 of membrane

Kit Contents:
Luminol/Enhancer, 50mL
Stable Peroxide Buffer, 50mL
100mL kit Product Instructions for product #34075 SuperSignal West Dura Extended Duration Substrate MSDS for product #34075 SuperSignal West Dura Extended Duration Substrate Certificate of Analysis for product #34075 SuperSignal West Dura Extended Duration Substrate $320.00

Add to cart
34075PROMO SuperSignal West Dura Extended Duration Substrate
Formulation: 4 x Part No. 34075

Promotional item; expires June 30, 2014
4 x 100mL kit $960.00

Add to cart
34076 SuperSignal West Dura Extended Duration Substrate
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 25 mini-gel blots; 2000cm2 of membrane

Kit Contents:
Luminol/Enhancer, 100mL
Stable Peroxide Buffer, 100mL
200mL kit Product Instructions for product #34076 SuperSignal West Dura Extended Duration Substrate MSDS for product #34076 SuperSignal West Dura Extended Duration Substrate Certificate of Analysis for product #34076 SuperSignal West Dura Extended Duration Substrate $535.00

Add to cart
34076PROMO SuperSignal West Dura Extended Duration Substrate
Formulation: 4 x Part No. 34076

Promotional item; expires June 30, 2014
4 x 200mL kit $1,605.00

Add to cart
34075AB SuperSignal West Dura Extended Duration Substrate
Formulation: Proprietary luminol and peroxide solutions

Kit Contents:
Luminol/Enhancer
Stable Peroxide Buffer
Custom MSDS for product #34075AB SuperSignal West Dura Extended Duration Substrate

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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