Thermo Scientific SuperSignal Molecular Weight Protein Ladders (20 to 150K) are a ready-to-use mix of recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems.
SuperSignal Molecular Weight Protein Ladders provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent, fluorescent, chromogenic or other detection systems. Two versions of the MW marker are offered. The regular formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. The enhanced ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1/50,000 (25ng/mL) to 1/100,000 (10ng/mL) dilution of secondary antibody with SuperSignal West Pico Chemiluminescent substrate.
- Ready-to-use – liquid format is ready to load and requires no boiling
- Direct visualization – see marker bands with gel stains and blotting detection reagents
- Consistent – even band development regardless of detection method
- Compatible – enhanced version for compatibility with mouse monoclonal primary antibodies
- Stable – store long term at -20°C, three months at 4°C or one month at ambient
The SuperSignal* Molecular Weight Protein Ladder and SuperSignal Enhanced Molecular Weight Protein Ladder contain ready-to-use stabilized mixtures of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. The MW marker mixes also contain a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers. The standards are compatible with nitrocellulose and PVDF membranes, multiple blocking buffers and many other detection methods.
|Reliable and even banding patterns regardless of detection method. SuperSignal Enhanced Molecular Weight Protein Ladders were separated by electrophoresis and detected by Western blotting and in-gel protein stains. (A.) For Western blot detection, the Protein Ladders were transferred to PVDF membrane, membrane was blocked with Thermo Scientific StartingBlock T20 (TBS) Blocking Buffer (Part No. 37543), incubated in a 1/1000 dilution of anti-Cdk5 (Labvision, mouse monoclonal antibody), and then incubated in a 1/5000 dilution of Thermo Scientific Goat anti-Mouse IgG, HRP-conjugated (Part No. 31430). The membrane was incubated in Thermo Scientific Pierce ECL Western Blotting Substrate (Part No. 32106) prior to film exposure. (B.) Thermo Scientific GelCode Blue Stain Reagent (Part No. 24590) and (C.) Thermo Scientific Pierce Silver Stain Kit (Part No. 24612).
SuperSignal Molecular Weight Protein Ladders may be used in most blotting applications. However, SuperSignal Enhanced Molecular Weight Protein Ladders are designed to boost results specifically with mouse monoclonal primary antibodies.
SuperSignal Molecular Weight Protein Ladders have flexible storage conditions depending on how often they are used. For intermittent use, store the molecular weight marker mix at -20°C. For labs performing gel electrophoresis on a routine basis, the protein standards can be kept at ambient temperature on the lab bench for one month or stored at 4°C for three months.
|Thermo Scientific SuperSignal Molecular Weight Protein Ladders outperform other chemiluminescent molecular weight protein standards. SuperSignal Molecular Weight Protein Ladders were compared to Invitrogen™ MagicMark™ XP Western Protein Standard in Western blots probed with mouse or rabbit primary antibodies.
(A.) 5µL of SuperSignal Enhanced Molecular Weight Protein Ladders and MagicMark XP Western Protein Standard were separated by electrophoresis, transferred to PVDF membrane and blocked with StartingBlock T20 (TBS) Blocking Buffer (Part No. 37543). The membrane was then probed with mouse monoclonal anti-Cdk5 (Labvision) at 1/1000 dilution followed by Goat anti-Mouse IgG, HRP-Conjugated (Part No. 31430) at 1/5000 dilution. Detection was performed with Pierce ECL Western Blotting Substrate (Part No. 32106) and exposed to film for 1 minute.
(B.) 10µL of SuperSignal Molecular Weight Protein Ladders and MagicMark XP Western Protein Standard were separated by electrophoresis, transferred to nitrocellulose membrane and blocked with nonfat dry milk in Tris-buffered saline with Tween™ 20. The membrane was then probed with Thermo Scientific Rabbit anti-S6 (Part No. PA1-968) at 1/2000 dilution followed by Donkey anti-Rabbit IgG, HRP-Conjugated (Part No. 31458) at 1/100,000 dilution. Detection was performed with Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080) and exposed to film for 30 seconds.
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