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SuperBlock Blocking Buffers


A biotin-free and serum-free blocking buffer with excellent stabilizing properties.

SuperBlock Blocking Buffers

Thermo Scientific SuperBlock Blocking Buffers are optimized PBS and TBS solutions containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

Blocking microplates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Highlights:

  • Fast – block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
  • Flexible – guaranteed to be biotin-free for use with streptavidin system
  • Convenience – available in multiple buffer formats (in PBS or TBS with or without Tween™ 20 detergent) and package sizes (1L, 5L and dry-blend pouches)
  • Low background – the non-serum protein solution yields a high signal-to-noise ratio
  • Stable – store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Product Details:

SuperBlock Blocking buffer performs better then non-fat dry milk in Western blots.
Thermo Scientific SuperBlock Blocking Buffer in PBS is better than milk for sensitive detection of target proteins. HeLa cell lysate (20, 10, 5, 2.5, 1.25, 0.625 and 0.3125µg) was separated by SDS-PAGE, and transferred to nitrocellulose (Panels A-C, Part No. 88014) or PVDF (Panels D-E, Part No. 88585) membrane. Membranes were blocked for one hour using 5% non-fat milk in Tris-buffered saline with 0.05% Tween™ 20 Detergent or SuperBlock Blocking Buffer in phosphate-buffered saline with 0.05% Tween 20 Detergent. Membranes were probed for the indicated targets. Blots were incubated in Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080) for 5 minutes and exposed to film (Part No. 34091).

References:

  1. Ikeda, K., et al. (2003). J. Biol. Chem. 278, 7725-7734.
  2. Leclerc, G.J. and Barredo, J.C. (2001). Clin. Cancer Res. 7, 942-951.
  3. Subbarayan, V., et al. (2001). Cancer Res. 61, 2720-276.
  4. Walters, R.W., et al. (2002). Cell 100, 789-799.

Related Resources:

Blocking buffers for Western blot and ELISA -- technical guide
Blocking strategies for immunohistochemistry -- technical guide

Related Products:

All Blocking Buffers

Note: Part No. 37517 and 37537 are specifically designed to be used for Western blotting with precipitating substrates. They are not intended for use with chemiluminescent substrates or microplates (ELISA).

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
37580 SuperBlock (PBS) Blocking Buffer
Formulation: Proprietary protein in phosphate-buffered saline (pH 7.4) containing Kathon Anti-microbial Agent
Sufficient For: 3 blots or microplates at 30mL each
100mL Product Instructions for product #37580 SuperBlock (PBS) Blocking Buffer MSDS for product #37580 SuperBlock (PBS) Blocking Buffer Certificate of Analysis for product #37580 SuperBlock (PBS) Blocking Buffer $36.00

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37515 SuperBlock (PBS) Blocking Buffer
Formulation: Proprietary protein formulation in phosphate-buffered saline, pH 7.4, with Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37515 SuperBlock (PBS) Blocking Buffer MSDS for product #37515 SuperBlock (PBS) Blocking Buffer Certificate of Analysis for product #37515 SuperBlock (PBS) Blocking Buffer $161.00

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37515B SuperBlock (PBS) Blocking Buffer
Formulation: Proprietary protein formulation in phosphate-buffered saline, pH 7.4, with Kathon preservative
Custom MSDS for product #37515B SuperBlock (PBS) Blocking Buffer Certificate of Analysis for product #37515B SuperBlock (PBS) Blocking Buffer
37518 SuperBlock (PBS) Blocking Buffer
Formulation: Proprietary protein formulation in phosphate-buffered saline, pH 7.4, with Kathon preservative
Sufficient For: 150 blots or microplates at 30mL each
5L Product Instructions for product #37518 SuperBlock (PBS) Blocking Buffer MSDS for product #37518 SuperBlock (PBS) Blocking Buffer Certificate of Analysis for product #37518 SuperBlock (PBS) Blocking Buffer $559.00

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37516 SuperBlock T20 (PBS) Blocking Buffer
Formulation: Proprietary protein formulation in phosphate-buffered saline, pH 7.4, with 0.05% Tween-20 and Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37516 SuperBlock T20 (PBS) Blocking Buffer MSDS for product #37516 SuperBlock T20 (PBS) Blocking Buffer Certificate of Analysis for product #37516 SuperBlock T20 (PBS) Blocking Buffer $176.00

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37517 SuperBlock (PBS) Blocking Buffer - Blotting
Formulation: Proprietary protein formulation in phosphate buffer, pH 7.4, with Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each


This formulation is optimized for use with precipitating chromogenic substrates.
1L Product Instructions for product #37517 SuperBlock (PBS) Blocking Buffer - Blotting MSDS for product #37517 SuperBlock (PBS) Blocking Buffer - Blotting Certificate of Analysis for product #37517 SuperBlock (PBS) Blocking Buffer - Blotting $146.00

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37581 SuperBlock (TBS) Blocking Buffer
Formulation: Proprietary protein in Tris-buffered saline (pH 7.4) containing Kathon Anti-microbial Agent
Sufficient For: 3 blots or microplates at 30mL each
100mL Product Instructions for product #37581 SuperBlock (TBS) Blocking Buffer MSDS for product #37581 SuperBlock (TBS) Blocking Buffer Certificate of Analysis for product #37581 SuperBlock (TBS) Blocking Buffer $36.00

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37535 SuperBlock (TBS) Blocking Buffer
Formulation: Proprietary protein formulation in Tris-buffered saline, pH 7.4, with Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37535 SuperBlock (TBS) Blocking Buffer MSDS for product #37535 SuperBlock (TBS) Blocking Buffer Certificate of Analysis for product #37535 SuperBlock (TBS) Blocking Buffer $161.00

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37535B SuperBlock (TBS) Blocking Buffer
Formulation: Proprietary protein formulation in Tris-buffered saline, pH 7.4, with Kathon preservative
Custom MSDS for product #37535B SuperBlock (TBS) Blocking Buffer
37545 SuperBlock (TBS) Blocking Buffer Dry Blend
Formulation: Powder prepared from proprietary protein formulation in Tris-buffered saline, pH 7.4
Sufficient For: 200mL (approx. 7 blots or microplates) per pack
5 packs Product Instructions for product #37545 SuperBlock (TBS) Blocking Buffer Dry Blend MSDS for product #37545 SuperBlock (TBS) Blocking Buffer Dry Blend Certificate of Analysis for product #37545 SuperBlock (TBS) Blocking Buffer Dry Blend $130.00

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37545B SuperBlock (TBS) Blocking Buffer Dry Blend
Formulation: Powder prepared from proprietary protein formulation in Tris-buffered saline, pH 7.4
Custom MSDS for product #37545B SuperBlock (TBS) Blocking Buffer Dry Blend
37536 SuperBlock T20 (TBS) Blocking Buffer
Formulation: Proprietary protein formulation in Tris-buffered saline, pH 7.4, with 0.05% Tween-20 and Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37536 SuperBlock T20 (TBS) Blocking Buffer MSDS for product #37536 SuperBlock T20 (TBS) Blocking Buffer Certificate of Analysis for product #37536 SuperBlock T20 (TBS) Blocking Buffer $176.00

Add to cart
37537 SuperBlock (TBS) Blocking Buffer - Blotting
Formulation: Proprietary protein formulation in Tris-buffered saline, pH 7.4, with Kathon preservative
Sufficient For: 30 blots at 30mL each


This formulation is optimized for use with precipitating chromogenic substrates.
1L Product Instructions for product #37537 SuperBlock (TBS) Blocking Buffer - Blotting MSDS for product #37537 SuperBlock (TBS) Blocking Buffer - Blotting Certificate of Analysis for product #37537 SuperBlock (TBS) Blocking Buffer - Blotting $146.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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