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SulfoLink Immobilization Kits and Coupling Resin


Immobilize sulfhydryl-containing peptides or proteins for affinity purification.

SulfoLink Coupling Resin

Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest. Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.

Highlights:

  • Specific to sulfhydryl (-SH) groups – iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
  • Kits for peptides or proteins – two kits include optimized reagents for preparing either peptide or protein samples for efficient immobilization
  • Fast – spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
  • Flexible coupling conditions – use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine•HCl), as needed for protein or peptide solubility during coupling reaction
  • Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization, and affinity purification
  • High capacity – Immobilize 1 to 2mg of peptide or 2 to 20mg of protein per 2mL column of SulfoLink Coupling Resin

Applications:

  • Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
  • Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

Product Details:

Peptide immobilization chemistry of SulfoLink Resin
Peptide immobilization chemistry for Thermo Scientific SulfoLink Coupling Resins. Sulfhydryl groups react with iodoacetyl groups to form covalent bonds. Learn more about sulfhydryl-reactive immobilization supports and about haloacetyl crosslinking chemistry.

More Product Data

References:

  1. Vacher H. et al. (2011) Cdk-mediated phosphorylation of the Kv{beta}2 auxiliary subunit regulates Kv1 channel axonal targeting. J. Cell Biol. 192, 813-24.
  2. Okamoto Y. and Shikano S. (2011) Phosphorylation-dependent c-terminal binding of 14-3-3 proteins promotes cell surface expression of HIV co-receptor GPR15. J. Biol. Chem. 286, 7171-81.
  3. Hoffer P. et al. (2011) Posttranscriptional gene silencing in nuclei. PNAS. 108, 409-14.
  4. Zhao B. et al. (2010) Nogo-B receptor is essential for angiogenesis in zebrafish via Akt pathway. Blood. 116, 5423-33.
  5. Yamanaka T. et al. (2010) Mutant huntingtin fragment selectively suppresses Brn-2 POU domain transcription factor to mediate hypothalamic cell dysfunction. Hum. Mol. Genet. 19, 2099-112.
  6. Liu J. et al. (2010) Membrane remodeling during reticulocyte maturation. Blood. 115, 2021-7.
  7. Nagy Z. et al. (2009) The human SPT20-containing SAGA complex plays a direct role in the regulation of endoplasmic reticulum stress-induced genes. Mol. Cell. Biol. 29, 1649-60.
  8. Chang J. et al. (2009) The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons. J. Cell Sci. 122, 2274-82.

Related Resources:

Review of affinity purification
Review of covalent immobilization methods
Sulfhydryl-reactive crosslinker chemistry

Related Products:

Bond-Breaker TCEP Solution, Neutral pH
Imject Maleimide Activated mcKLH Kit
UltraLink Iodoacetyl Resin
Micro-scale Peptide Coupling Kit
Pierce Centrifuge Columns


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
44995 SulfoLink Immobilization Kit for Proteins, 2mL
Formulation: Multi-component kit
Sufficient For: Immobilizing 1 to 10mg of antibody per column

Kit Contents:
SulfoLink Columns, 2mL, 5 columns
SulfoLink Preparation Buffer, 7.5mL
SulfoLink Coupling Buffer, 500mL
Wash Solution, 120mL
PBS Pack (makes 500mL), 1 pack
2-Mercaptoethylamine·HCl, 5 x 6mg
L-Cysteine·HCl, 100mg
Zeba Spin Desalting Columns, 5mL, 5 columns
Column Accessories
5-column kit Product Instructions for product #44995 SulfoLink Immobilization Kit for Proteins, 2mL MSDS for product #44995 SulfoLink Immobilization Kit for Proteins, 2mL Certificate of Analysis for product #44995 SulfoLink Immobilization Kit for Proteins, 2mL $235.00

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44999 SulfoLink Immobilization Kit for Peptides, 2mL
Formulation: Multi-component kit
Sufficient For: Immobilizing 0.1 to 1mg of sulfhydryl-peptide per column

Kit Contents:
SulfoLink Columns, 2mL, 5 columns
SulfoLink Coupling Buffer, 120mL
Wash Solution, 120mL
PBS Pack (makes 500mL), 1 pack
Bond-Breaker TCEP Solution, Neutral pH, 0.5mL
L-Cysteine·HCl, 100mg
Column Accessories
5-column kit Product Instructions for product #44999 SulfoLink Immobilization Kit for Peptides, 2mL MSDS for product #44999 SulfoLink Immobilization Kit for Peptides, 2mL Certificate of Analysis for product #44999 SulfoLink Immobilization Kit for Peptides, 2mL $287.00

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20325 SulfoLink Immobilization Trial Kit, 2mL
Formulation: Multi-component kit
Sufficient For: Immobilizing 1mg of peptide or 10mg protein per column

Kit Contents:
SulfoLink Column, 2mL, 1 column
SulfoLink Preparation Buffer, 7.5mL
SulfoLink Coupling Buffer, 120mL
Wash Solution, 25mL
PBS Pack (makes 500mL), 1 pack
2-Mercaptoethylamine·HCl, 6mg
Bond-Breaker TCEP Solution, Neutral pH, 0.5mL
L-Cysteine·HCl, 100mg
Zeba Spin Desalting Column, 5mL, 1 column
Column Accessories
1-column kit Product Instructions for product #20325 SulfoLink Immobilization Trial Kit, 2mL MSDS for product #20325 SulfoLink Immobilization Trial Kit, 2mL Certificate of Analysis for product #20325 SulfoLink Immobilization Trial Kit, 2mL $177.00

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20401 SulfoLink Coupling Resin
Formulation: Iodoacetyl-activated crosslinked 6% beaded agarose, slurried in 50% glycerol, 10mM EDTA with sodium azide
Sufficient For: Immobilizing 1mg peptide or 10mg antibody per mL of resin
10mL Product Instructions for product #20401 SulfoLink Coupling Resin MSDS for product #20401 SulfoLink Coupling Resin Certificate of Analysis for product #20401 SulfoLink Coupling Resin $157.00

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20402 SulfoLink Coupling Resin
Formulation: Iodoacetyl-activated crosslinked 6% beaded agarose, slurried in 50% glycerol, 10mM EDTA with sodium azide
Sufficient For: Immobilizing 1mg peptide or 10mg antibody per mL of resin
50mL Product Instructions for product #20402 SulfoLink Coupling Resin MSDS for product #20402 SulfoLink Coupling Resin Certificate of Analysis for product #20402 SulfoLink Coupling Resin $515.00

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20402B SulfoLink Coupling Resin
Formulation: Iodoacetyl-activated crosslinked 6% beaded agarose, slurried in 50% glycerol, 10mM EDTA with sodium azide
Sufficient For: Immobilizing 1mg peptide or 10mg antibody per mL of resin
Custom MSDS for product #20402B SulfoLink Coupling Resin Certificate of Analysis for product #20402B SulfoLink Coupling Resin
20404 SulfoLink Coupling Resin
Formulation: Iodoacetyl-activated crosslinked 6% beaded agarose, slurried in 50% glycerol, 10mM EDTA with sodium azide
Sufficient For: Immobilizing 1mg peptide or 10mg antibody per mL of resin
250mL Product Instructions for product #20404 SulfoLink Coupling Resin MSDS for product #20404 SulfoLink Coupling Resin Certificate of Analysis for product #20404 SulfoLink Coupling Resin $2,035.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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