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Premium Grade Sulfo-SMCC


Thoroughly tested, highly characterized and fully documented batches of reagent.

Pierce Premium Grade Sulfo-SMCC

Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of this popular amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-SMCC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-SMCC is a water-soluble, non-cleavable and membrane-impermeable amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms). NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to nearly 10mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Highlights:

  • Sulfo-SMCC – a water-soluble amine-to-sulfhydryl crosslinker with a stabilizing cyclohexane spacer arm
  • High quality – identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity – enhanced level of testing and characterization compared to standard grade
  • Lot retention – ample supply of past lots retained to ensure future process testing
  • Change management – Change Control Notification (CCN) service
  • Consistent manufacture – batch-specific manufacturing documentation review

Applications:

  • Enzyme labeling of antibodies - both enzyme and antibody specificity can be preserved
  • Create specific bioconjugates via one- or two-step crosslinking reactions
  • Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens

Product Details:

Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate; Sulfo-SMCC
 
Chemical structure of Sulfo-SMCC crosslinking reagent. For more complete information about the chemical properties and uses of this reagent, see the product page for standard grade Sulfo-SMCC.

 

Specifications of Premium Grade Sulfo-SMCC. We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Test Premium Grade Specification Standard Grade
Purity Greater than 90% by quantitative NMR using an internal standard (the highest standard for crosslinker purity) Yes
Visual White to off-white powder, free of foreign material Yes
Solubility Sample dissolves at 10mg/mL in DI water to give a clear, colorless solution;
Sample dissolves at 10mg/mL in 50mM sodium phosphate, pH 7.0 to give a clear, colorless solution
Yes
Identity IR scan shows only peaks characteristic of the structure and functional groups of Sulfo-SMCC crosslinker Yes
Mass identity Molecular weight of 436 m/z by mass spectrometry
Water content Karl Fischer titration, ≤ 2%
Trace metals All metals, including lead, ≤ 50 ppm
Elemental analysis Reported values for C, H, N, O, and S

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Premium Grade Reagents – complete menu
Sulfo-SMCC (standard grade)
Crosslinkers at a glance (selection guide)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
PG82085 Premium Grade Sulfo-SMCC
Formulation: Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate; powder solid
Sufficient For: 229mL of typical 1X (1mM) reaction solution
100mg Product Instructions for product #PG82085 Premium Grade Sulfo-SMCC MSDS for product #PG82085 Premium Grade Sulfo-SMCC Certificate of Analysis for product #PG82085 Premium Grade Sulfo-SMCC $438.00

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PG82086 Premium Grade Sulfo-SMCC
Formulation: Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate; powder solid
Sufficient For: 2.29L of typical 1X (1mM) reaction solution
1g Product Instructions for product #PG82086 Premium Grade Sulfo-SMCC MSDS for product #PG82086 Premium Grade Sulfo-SMCC Certificate of Analysis for product #PG82086 Premium Grade Sulfo-SMCC $2,940.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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