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Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent)

Ideal for selectively radiolabeling cell surface proteins with iodine-125.

Pierce Sulfo-SHPP (water-soluble Bolton-Hunter Reagent)

Thermo Scientific Pierce Sulfo-SHPP (water-soluble Bolton-Hunter Reagent) conjugates tyrosine-like functional groups to primary amines to increase the number of tyrosyl groups that can be iodinated by iodine-125 labeling procedures.


  • Also known as water-soluble Bolton Hunter Reagent, sulfosuccinimidyl-3 -(4-hydroxypheynyl) propionate
  • Iodinate before or after coupling Sulfo-SHPP to the molecule of interest
  • Useful for introducing tyrosyl groups on proteins sensitive to small amounts of organic solvent
  • Useful for labeling cell surfaces without exposing the cells to membrane permeable solvents

Product Details:

Chemical structure of Sulfo-SHPP, Bolton-Hunter Reagent Chemical structure of Thermo Scientific Pierce Sulfo-SHPP. Also known as water-soluble Bolton-Hunter Reagent, this compound conjugates to primary amines via its NHS-ester moiety to add tyrosine-like groups to peptides and proteins that can then be labeled with radioactive iodine (125I). For more information, see our review of NHS Ester Reaction Chemistry.


  1. Thompson, J.A., Lau, A.L. and Cunningham, D.D. (1987). Selective radiolabeling of cell surface proteins to a high specific activity. Biochem. 26, 743-750.
  2. Thirkell, D., Myles, A.D. and Russell, W.C. (1989). Serotype-8 and serocluster-specific surface-expressed antigens of Ureaplasma urealyticum. Infection and Immunity 57, 1697-1701.

Related Products:

Pierce Iodination Beads
Pierce Iodination Tubes
Pierce Iodination Reagent

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent)
Formulation: N-Sulfosuccinimidyl-3-(4-hydroxyphenyl)proprionate
Sufficient For: Approx. 50 uses to label a total of 1825mg of IgG using typical conditions
100mg Product Instructions for product #27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent) MSDS for product #27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent) Certificate of Analysis for product #27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent) $336.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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