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Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent)


Ideal for selectively radiolabeling cell surface proteins with iodine-125.

Pierce Sulfo-SHPP (water-soluble Bolton-Hunter Reagent)

Thermo Scientific Pierce Sulfo-SHPP (water-soluble Bolton-Hunter Reagent) conjugates tyrosine-like functional groups to primary amines to increase the number of tyrosyl groups that can be iodinated by iodine-125 labeling procedures.

Highlights:

  • Also known as water-soluble Bolton Hunter Reagent, sulfosuccinimidyl-3 -(4-hydroxypheynyl) propionate
  • Iodinate before or after coupling Sulfo-SHPP to the molecule of interest
  • Useful for introducing tyrosyl groups on proteins sensitive to small amounts of organic solvent
  • Useful for labeling cell surfaces without exposing the cells to membrane permeable solvents

Product Details:

Chemical structure of Sulfo-SHPP, Bolton-Hunter Reagent Chemical structure of Thermo Scientific Pierce Sulfo-SHPP. Also known as water-soluble Bolton-Hunter Reagent, this compound conjugates to primary amines via its NHS-ester moiety to add tyrosine-like groups to peptides and proteins that can then be labeled with radioactive iodine (125I). For more information, see our review of NHS Ester Reaction Chemistry.

References:

  1. Thompson, J.A., Lau, A.L. and Cunningham, D.D. (1987). Selective radiolabeling of cell surface proteins to a high specific activity. Biochem. 26, 743-750.
  2. Thirkell, D., Myles, A.D. and Russell, W.C. (1989). Serotype-8 and serocluster-specific surface-expressed antigens of Ureaplasma urealyticum. Infection and Immunity 57, 1697-1701.

Related Products:

Pierce Iodination Beads
Pierce Iodination Tubes
Pierce Iodination Reagent


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent)
Formulation: N-Sulfosuccinimidyl-3-(4-hydroxyphenyl)proprionate
Sufficient For: Approx. 50 uses to label a total of 1825mg of IgG using typical conditions
100mg Product Instructions for product #27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent) MSDS for product #27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent) Certificate of Analysis for product #27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent) $300.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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