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Sulfo-SANPAH Photoreactive Crosslinker

A long-arm, water-soluble, amine-reactive and light-activatable crosslinker based on aryl azide chemistry.

Pierce Sulfo-SANPAH

Thermo Scientific Pierce Sulfo-SANPAH is a long-arm (18.2 angstrom) crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.


  • Reactive groups: Sulfo-NHS ester and nitrophenyl azide
  • Reactive towards: amino groups and any nucleophile
  • Non-cleavable
  • N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate
  • Optimal photolysis occurs at 320-350 nm; minimizes damage to biomolecules by irradiation
  • Water soluble; non-cleavable

Product Details:

Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate; Sulfo-SANPAH
Chemical structure of Sulfo-SANPAH crosslinking reagent.


  1. Tiberi, M., et al. (1996). J. Biol. Chem. 271(7), 3771-3778.
  2. Wood, C.L. and O’Dorisio, M.S. (1985). Covalent crosslinking of vasoactive intestinal polypeptide to its receptors on intact human lymphoblasts. J. Biol. Chem. 260, 1243-1247. (HSAB example)

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Sulfo-LC-SDA – more efficient diazirine analog of Sulfo-SANPAH
Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22589 Sulfo-SANPAH
Formulation: Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate; powder solid
Sufficient For: 102mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22589 Sulfo-SANPAH MSDS for product #22589 Sulfo-SANPAH Certificate of Analysis for product #22589 Sulfo-SANPAH $230.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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