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Premium Grade Sulfo-NHS-SS-Biotin


Thoroughly tested, highly characterized and fully documented batches of reagent.

Premium Grade Sulfo-NHS-SS-Biotin

Thermo Scientific Pierce Premium Grade Sulfo-NHS-SS-Biotin is our highest quality formulation of this amine-reactive, cleavable biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-NHS-SS-Biotin provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines. This reagent is water-soluble, enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion. The compound is particularly useful for labeling and purifying cell surface proteins, because (a) its sulfonate group prevents it from permeating cell membranes and (b) its cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns.

Highlights:

  • Sulfo-NHS-SS-Biotin – for cleavable attachment of biotin to protein and cell surface amines
  • High quality – identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity – enhanced level of testing and characterization compared to standard grade
  • Lot retention – ample supply of past lots retained to ensure future process testing
  • Change management – Change Control Notification (CCN) service
  • Consistent manufacture – batch-specific manufacturing documentation review

Product Details:

Chemical structure of Sulfo-NHS-SS-Biotin
Chemical structure of Sulfo-NHS-SS-Biotin. This biotinylation reagent labels antibodies and other proteins and molecules that contain primary amines (e.g., side-chain of lysine). For more information, see the product page for standard grade Sulfo-NHS-SS-Biotin or our review of NHS Ester Reaction Chemistry.

 

Specifications of Premium Grade Sulfo-NHS-SS-Biotin. We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Test Premium Grade Specification Standard Grade
Purity Greater than 80% by quantitative NMR using an internal standard Yes
Visual White to light tan powder, free of foreign material Yes
Solubility Sample dissolves at 10mg/mL in water to give a clear, colorless solution
Sample dissolves at 10mg/mL in DMF to give a clear, colorless solution
Yes
Identity IR scan shows only peaks characteristic of Sulfo-NHS-SS-Biotin Yes
Mass identity Molecular weight of 606 m/z by mass spectrometry
Water content Karl Fischer titration, ≤ 2%
Trace metals All metals, including lead, ≤ 50 ppm;
Reported value for iron
Elemental analysis Reported values for C, H, N, O, and S

Related Resources:

Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)

Related Products:

Premium Grade Reagents – complete menu
Sulfo-NHS-SS-Biotin (standard grade)
Pierce Cell Surface Protein Isolation Kit
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins

 

Currently available by special order only.

Please contact our Large Volume and Custom Sales Department:
LVC.rockford@thermofisher.com
815-968-0747 ext. 300 or +800-874-3723 ext. 300

Request a bulk quote

 

 

 

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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