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Premium Grade Sulfo-NHS


Thoroughly tested, highly characterized and fully documented batches of reagent.

Pierce Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (–COOH) for conjugation with primary amines (–NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

Highlights:

  • Sulfo-NHS – use with EDC crosslinker to prepare amine-reactive NHS ester reagents
  • High quality – identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity – enhanced level of testing and characterization compared to standard grade
  • Lot retention – ample supply of past lots retained to ensure future process testing
  • Change management – Change Control Notification (CCN) service
  • Consistent manufacture – batch-specific manufacturing documentation review

Applications:

  • Improve efficiency of EDC coupling reactions
  • Convert carboxyls to amine-reactive sulfo-NHS esters
  • Membrane-impermable for cell surface labeling
  • Crosslink proteins to carboxyl-coated beads or surfaces more efficiently
  • Activate nanoparticles with amine-reactive sulfo-NHS esters

Product Details:

N-Hydroxysulfosuccinimide; Sulfo-NHS
Chemical structure of Sulfo-NHS. For more complete information about the chemical properties and uses of this reagent, see the product page for standard grade sulfo-NHS.

 

Specifications of Premium Grade Sulfo-NHS. We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Test Premium Grade Specification Standard Grade
Purity Greater than 95% by quantitative NMR using an internal standard (the highest standard for crosslinker purity); average lot purity is greater than 99% Yes
Visual White to light tan powder, free of foreign material Yes
Solubility Sample dissolves at 2mg/mL in deionized water to yield a clear, colorless solution Yes
Identity IR scan shows only peaks characteristic of N-hydroxysulfosuccinimide Yes
Mass identity Molecular weight of 217 m/z by mass spectrometry
Water content Karl Fischer titration, ≤ 2%
Trace metals All metals, including lead, ≤ 50 ppm
Elemental analysis Reported values for C, H, N, O, and S

 

Purity of Sulfo-NHS tested by quantitative NMR
 

Greater than 99% pure Sulfo-NHS. Prepared batches of Pierce Premium Grade Sulfo-NHS are tested by quantitative NMR against an internal standard to ensure purity and lot-to-lot consistency. Each peak in this typical NMR scan corresponds to a particular proton of sulfo-NHS, the internal standard, or the solvents used in the NMR analysis.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

NHS and Sulfo-NHS FAQ
Overview of crosslinking
Chemistry of crosslinking

Related Products:

Premium Grade Reagents – complete menu
Sulfo-NHS (standard grade)
EDC (Premium Grade)
EDC (standard grade)
Crosslinkers at a glance (selection guide)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
PG82071 Premium Grade Sulfo-NHS
Formulation: N-Hydroxysulfosuccinimide; powder solid
Sufficient For: 0.46L of typical 1X (5mM) reaction solution
500mg Product Instructions for product #PG82071 Premium Grade Sulfo-NHS MSDS for product #PG82071 Premium Grade Sulfo-NHS Certificate of Analysis for product #PG82071 Premium Grade Sulfo-NHS $191.00

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PG82072 Premium Grade Sulfo-NHS
Formulation: N-Hydroxysulfosuccinimide; powder solid
Sufficient For: 9.21L of typical 1X (5mM) reaction solution
10g Product Instructions for product #PG82072 Premium Grade Sulfo-NHS MSDS for product #PG82072 Premium Grade Sulfo-NHS Certificate of Analysis for product #PG82072 Premium Grade Sulfo-NHS $1,913.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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