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Sulfo-NHS-Acetate (Sulfosuccinimidyl Acetate)

A water-soluble reagent for blocking primary amines.

Pierce Sulfo-NHS-Acetate

Thermo Scientific Pierce Sulfo-NHS-Acetate is a small compound used for blocking primary amines (e.g., lysine side chains). This compound forms stable, covalent amide bonds with amine groups to prevent specific interactions or conjugations.

Sulfo-NHS-Acetate is typically used to prevent polymerization when performing protein crosslinking reactions and when conjugating peptides to carrier proteins for immunogen production. Blocking amines on the peptide allows directed conjugation of carboxylic acids on the peptide to primary amines on the protein using EDC.


  • Irreversibly block primary amines
  • Perform reactions in standard non-amine-containing buffers at pH 7 or greater
  • Prevent polymerization when performing protein crosslinking reactions

Product Details:

Sulfosuccinimidyl acetate; Sulfo-NHS-acetate

Structure and properties of sulfo-NHS-acetate:

  • Functional groups: sulfo-NHS ester (amine-reactive)
  • Chemical name: sulfo-N-hydroxysulfosuccinimide acetate
  • Chemical formula: C6H6O7NSNa
  • Molecular weight: 259.17
  • Form: white to off-white powder, free of foreign material
  • Solubility: 10mM produces a clear and colorless solution

Related Resources:

Overview of crosslinking and protein modification
Block amino groups to prevent polymer formation in peptide-carrier protein conjugations (Tech Tip #18)

Related Products:

Citraconic Anhydride – reversibly block primary amines
TFCS – block and extend primary amines
MS(PEG)n – block and pegylated primary amines

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
26777 Sulfo-NHS-Acetate
Formulation: Sulfosuccinimidyl acetate; powder solid
Sufficient For: 386mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
100mg Product Instructions for product #26777 Sulfo-NHS-Acetate MSDS for product #26777 Sulfo-NHS-Acetate Certificate of Analysis for product #26777 Sulfo-NHS-Acetate $142.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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