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Sulfo-LC-SPDP


A water-soluble, long-chain disulfide crosslinker for reversible amine-to-sulfhydryl conjugation.

Pierce Sulfo-LC-SPDP

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

Sulfo-LC-SPDP is Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

Highlights:

  • Reactive groups: NHS ester and pyridyldithiol
  • Reactive towards: amino and sulfhydryl groups
  • Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
  • Water soluble, sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50mM DTT or TCEP at pH 8.5
  • Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
  • Cleavable crosslinker allows separation of crosslinked products
  • Sulfo-LC-SPDP crosslinker is not membrane permeable, allowing cell surface labeling
  • Compare to other varieties of SPDP-type reagents, including pegylated forms

Applications:

  • Reversible crosslinking of proteins
  • Adding sulfhydryl groups to proteins by attachment to lysine residues
  • Protein crosslinking to create antibody-enzyme and other bioconjugates via a two-step reaction
  • Attaching haptens to carrier proteins for antibody production
  • Preparing immunotoxin conjugates

Product Details:

Sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate; Sulfo-LC-SPDP
 
Chemical structure of Sulfo-LC-SPDP crosslinking reagent.

 

Properties of Sulfo-LC-SPDP.
Molecular formula C18H22O8N3S3Na
Molecular weight 527.57
Spacer arm length 15.7 Å
Storage conditions -20°C, protect from moisture, use only fresh solutions
Reactive groups: Sulfo-NHS ester, reacts with primary amines
  Pyridyl disulfide, reacts with sulfhydryls

Specifications:

We manufacture Sulfo-LC-SPDP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-LC-SPDP is tested to meet the following minimum specifications.

  • Identity: IR scan shows only peaks characteristic of the structure and functional groups of Sulfo-LC-SPDP crosslinker
  • Solubility: ≥ 95mg/mL in DI water
  • Purity: ≥ 90% by quantitative NMR (the highest standard for crosslinker purity)

References:

  1. Carlsson, J., et al. (1978) Biochem. J. 173, 723-737.
  2. Stan, R.V. (2004) Am. J. Physio.l Heart Circ. Physiol. 286, H1347-H1353.
  3. Mader, C., et al. (2004) J. Bacteriol.186, 1758-1768.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
Other SPDP-type Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21650 Sulfo-LC-SPDP
Formulation: Sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate; powder solid
Sufficient For: 95mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #21650 Sulfo-LC-SPDP MSDS for product #21650 Sulfo-LC-SPDP Certificate of Analysis for product #21650 Sulfo-LC-SPDP $379.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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