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A water-soluble amine-to-sulfhydryl crosslinker with a medium-length spacer arm (9.4 angstroms).

Pierce Sulfo-EMCS

Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).


  • Reactive groups: sulfo-NHS ester and maleimide
  • Reactive towards: amino and sulfhydryl groups
  • Sulfo-NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
  • Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
  • Non-cleavable
  • Water-soluble (compare to EMCS)
  • Aliphatic spacer offers low potential for eliciting an immune response

Product Details:

N-epsilon-Maleimidocaproyl-oxysulfosuccinimide ester; Sulfo-EMCS
Chemical structure of Sulfo-EMCS crosslinking reagent.


  1. Fujiwara, K., et al. (1988). J. Immunol. Methods 112, 77-83.
  2. Peeters, J.M., et al. (1989). J. Immunol. Methods 120, 133-143.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22307 Sulfo-EMCS
Formulation: N-epsilon-Maleimidocaproyl-oxysulfosuccinimide ester; powder solid
Sufficient For: 122mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22307 Sulfo-EMCS MSDS for product #22307 Sulfo-EMCS Certificate of Analysis for product #22307 Sulfo-EMCS $267.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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