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Sulfo-EGS (Ethylene glycol bis[sulfosuccinimidylsuccinate])


The water-soluble form of hydroxylamine-cleavable EGS crosslinker.

Pierce Sulfo-EGS

Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Highlights:

  • Reactive groups: sulfo-NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Water-soluble; compare to EGS
  • Membrane-impermeable, allowing for cell surface labeling
  • Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
  • Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product Details:

Ethylene glycol bis(sulfosuccinimidylsuccinate); Sulfo-EGS
 
Chemical structure of Sulfo-EGS crosslinking reagent.

References:

  1. Browning, J. and Ribolini, A. (1989). Studies on the differing effects of tumor necrosis factor and lymphotoxin on the growth of several human tumor lines. J. Immunol. 143, 1859-1867. (EGS example)
  2. Dihazi, G.H. and Sinz, A. (2003). Rapid Commun. Mass Spectrom. 17, 2005-2014.
  3. Sinz, A. (2003). J. Mass Spectrom. 38, 1225-1237.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
EGS (membrane permeable)
Hydroxylamine-HCl


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21566 Sulfo-EGS
Formulation: Ethylene glycol bis(sulfosuccinimidylsuccinate); powder solid
Sufficient For: 76mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #21566 Sulfo-EGS MSDS for product #21566 Sulfo-EGS Certificate of Analysis for product #21566 Sulfo-EGS $143.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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