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Sulfo-EGS (Ethylene glycol bis[sulfosuccinimidylsuccinate])

The water-soluble form of hydroxylamine-cleavable EGS crosslinker.

Pierce Sulfo-EGS

Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.


  • Reactive groups: sulfo-NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Water-soluble; compare to EGS
  • Membrane-impermeable, allowing for cell surface labeling
  • Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
  • Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product Details:

Ethylene glycol bis(sulfosuccinimidylsuccinate); Sulfo-EGS
Chemical structure of Sulfo-EGS crosslinking reagent.


  1. Browning, J. and Ribolini, A. (1989). Studies on the differing effects of tumor necrosis factor and lymphotoxin on the growth of several human tumor lines. J. Immunol. 143, 1859-1867. (EGS example)
  2. Dihazi, G.H. and Sinz, A. (2003). Rapid Commun. Mass Spectrom. 17, 2005-2014.
  3. Sinz, A. (2003). J. Mass Spectrom. 38, 1225-1237.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
EGS (membrane permeable)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
21566 Sulfo-EGS
Formulation: Ethylene glycol bis(sulfosuccinimidylsuccinate); powder solid
Sufficient For: 76mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #21566 Sulfo-EGS MSDS for product #21566 Sulfo-EGS Certificate of Analysis for product #21566 Sulfo-EGS $147.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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