The Thermo Scientific Pierce Substrate Buffers include optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
- Minimize assay-to-assay variability
Buffered stable hydrogen peroxide (H2O2) is used by researchers who prefer to make their own HRP-ELISA substrates. This is easily done by adding a chromogen of choice to the buffer. Our Stable Peroxide Substrate Buffer has a long shelf life (12 months after receipt) and is provided as a 10-fold concentrate. A total volume of 1000mL can be made from one 100mL bottle of concentrate. If you use 10mL of substrate per plate, this will allow you to make substrate for 100 plates from only one bottle of Stable Peroxide Substrate Buffer.
Diethanolamine Substrate Buffer is used with soluble alkaline phosphatase substrates such as PNPP. Our formulation is convenient, ready-to-use and reduces the possibility of assay-to-assay variability. Diethanolamine Substrate Buffer is sold as 5X concentrate with an optimal, consistent pH. It is stable even at a 1X concentration.
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Assay development and ELISA technical guide
ELISA substrate selection guide