Thermo Scientific Pierce Strong Cation and Anion Exchange Spin Columns enable rapid protein fractionation and sample preparation based on charge differences.
Pierce Strong Ion Exchange Spin Columns use membrane-adsorption as a chromatographic method to fractionate proteins based on their charge differences. The matrix has a highly porous structure with pores larger than 3000nm, providing proteins easy access to the membrane’s charged ligands. Therefore, adsorptive membranes maintain high efficiencies at high-flow rates and when fractionating large biomolecules with small diffusivities.
- Fast and simple – membrane-based spin format eliminates column packing
- Convenient and expandable – centrifugal format enables convenient processing of multiple samples in parallel
- Robust – membrane adsorber spin columns do not crack or run dry
- Low bed volume – small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions
- Mini Format: for binding up to 4mg (500µL capacity)
- Maxi Format: for binding up to 80mg (20mL capacity)
- Pre-fractionation of proteins in lysate
- Scouting purification conditions for new protein preparation protocols
- Removal of endotoxins from monoclonal antibodies
- Polishing His-tagged proteins after metal chelate chromatography
- Purification and concentration of proteins
- Purification of antibodies from serum, ascites or tissue culture supernatant
- Removal of detergent from protein solutions
- Sample preparation before 1D or 2D PAGE
- Purification of phosphopeptides before MS analysis
|Protocol summary for Thermo Scientific Pierce Strong Cation and Anion Ion Exchange Spin Columns.
Ion exchange chromatography separates molecules based on differences in their accessible surface charges. This technique is widely used in the pre-fractionation or purification of a target protein from crude biological samples. Novel membrane-based ion exchange chromatography is attracting attention because of its advantages over resin-based column chromatography. Membrane-based ion exchange chromatography has shorter diffusion times than resin-based chromatography. Interactions between molecules and active sites on the membrane occur in a convective manner through pores, which shortens the diffusion time compared with fluid inside the pores of a resin particle. Also, the relatively mild binding and eluting conditions of this separation method produce high protein recovery with intact biological activity.
Fe-NTA Phosphopeptide Enrichment Kit
C18 Spin columns
Cell Lysis Reagents for Protein Extraction
Subcellular Fractionation Kits